小麦Hussar衍生品系抗叶锈病基因分析  

Location of Resistance to Leaf Rust in Wheat Line Derived from Hussar

作　　者：赵欣雨 王羽[2] 李艳艳 高维芳 李星[1] 康占海[1] ZHAO Xinyu;WANG Yu;LI Yanyan;GAO Weifang;LI Xing;KANG Zhanhai(College of Plant Protection,Hebei Agricultural University,Baoding 071000;College of Food Science and Technology,Hebei Agricultural University,Baoding 071000)

机构地区：[1]河北农业大学植物保护学院,保定071000 [2]河北农业大学食品科技学院,保定071000

出　　处：《植物遗传资源学报》2024年第4期622-628,共7页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(32001890);2023年度河北省引进留学人员资助项目(C20230107)。

摘　　要：以抗叶锈病小麦品系Hussar的衍生品系H103P为抗病亲本,郑州5389为感病亲本杂交得到的234个F4家系群体为材料,进行抗叶锈病基因定位分析。利用带有不同毒力的16个叶锈菌生理小种进行苗期抗叶锈性鉴定,结果表明周麦22及携带Lr13、Lr23和Lr16单基因的载体品种对16个叶锈菌生理小种均表现感病,H103P对除PHKT外的所有小种表现抗病,表明H103P抗叶锈性与携带Lr13、Lr23和Lr16单基因的载体品种不同。利用5种强毒力混合菌种(THTT、PHTT^(②)、FHJS^(②)、PHKS、PHTT^(①))进行田间抗叶锈性鉴定,结果表明H103P、SAAR、周麦22以及Lr13载体品种田间表现均为高抗,234个F4家系群体抗性呈连续性分布,在田间表现出良好的成株期抗性。抗叶锈病基因定位分析结果表明,在小麦品系H103P中定位到1个位于小麦2BS染色体上的抗叶锈病基因,暂命名为LrHu。利用含有Lr13的特异性引物对H103P和郑州5389的扩增产物进行特异性酶切,结果发现小麦品系H103P含有抗叶锈病基因Lr13。小麦抗叶锈病基因LrHu与Lr13的关系还需进一步研究验证。This study used the derivative line H103P of the wheat line Hussar,which has resistance to leaf rust,as the resistant parent and Zhengzhou 5389 as the susceptible parent to hybridize and obtain 234 F4 family populations as materials for leaf rust resistance gene mapping analysis.Identification of leaf rust resistance during seedling stage using 16 physiological races of leaf rust fungus with different virulence.The results showed that Zhoumai 22 and vector varieties carrying Lr13,Lr23 and Lr16 single gene were susceptible to 16 physiological races of leaf rust.Except for one race(PHKT),H103P showed resistance to all other races.The resistance of H103P is different from the vector varieties corresponding to these three genes(Lr13,Lr23 and Lr16).Use 5 highly virulent mixed strains(THTT,PHTT^(②),FHJS^(②),PHKS,PHTT^(①))for field identification of leaf rust resistance.The results showed that H103P,SAAR,Zhoumai 22 and Lr13 vector variety exhibited high resistance in the field.234 F4 family populations are distributed continuously and exhibit good resistance during the mature stage in the field.The results of leaf rust resistance gene mapping analysis indicate that H103P is located as a disease resistance gene on the 2BS chromosome of wheat,temporarily named LrHu.Using specific primers containing Lr13 for specific cleavage of H103P and amplification products of Zhengzhou 5389,it is showed that the wheat leaf rust resistance gene Lr13 is contained in H103P.The relationship between the wheat leaf rust resistance gene LrHu and Lr13 is needed to verify by the further research.

关 键 词：小麦叶锈病 主效基因定位 分子标记 

分 类 号：S512.1[农业科学—作物学]