柔嫩艾美耳球虫热休克蛋白90和组蛋白4对DF-1细胞凋亡的影响  

Effect of Eimeria tenella Heat Shock Protein 90 and Histone 4 on Apoptosis in DF-1 Cells

作　　者：陈玲玲 王黎霞[2] 葛玉杰 王梦琦 尹徽 李治瀚 郭璐嘉 张建军[1] 安健[1] CHEN Lingling;WANG Lixia;GE Yujie;WANG Mengqi;YIN Hui;LI Zhihan;GUO Lujia;ZHANG Jianjun;AN Jian(College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,China;College of Animal Science and Veterinary Medicine,Beijing Vocatioeal College of Agriculture,Beijing 102442,China)

机构地区：[1]北京农学院动物科学技术学院,北京昌平102206 [2]北京农业职业学院畜牧兽医系,北京房山102442

出　　处：《中国兽医杂志》2024年第7期71-76,共6页Chinese Journal of Veterinary Medicine

基　　金：北京市特色高水平骨干专业群项目-动物医学专业群项目-技术平台与社会服务建设项目(PXM2020-157102-000060-15);北京农业职业学院院级科研项目(XY-JT-03);北京农学院教改项目(BUA2021 JG44)。

摘　　要：为了探究柔嫩艾美耳球虫热休克蛋白90(EtHSP90)和组蛋白(EtH4)在体外对鸡胚成纤维细胞(DF-1细胞)凋亡的影响,本试验构建真核荧光表达质粒pEGFP-N1-EtHSP90和pEGFP-N1-EtH4并转染至DF-1细胞,用荧光显微镜观察转染效果,Western blot验证蛋白表达,流式细胞术检测DF-1细胞凋亡。结果显示,经验证成功构建重组质粒pEGFP-N1-EtHSP90和pEGFP-N1-EtH4。流式细胞术检测发现,pEGFP-N1-EtHSP90组早期凋亡率显著低于空白组、pEGFP-N1组和pEGFP-N1-EtH4组(P<0.05);pEGFP-N1-EtH4组早期凋亡率与空白组和pEGFP-N1组相比差异不显著(P>0.05)。因此,EtHSP90早期可抑制细胞凋亡,EtH4既不促进细胞凋亡,也不抑制细胞凋亡。本试验结果为后续深入探究2个蛋白的凋亡机制提供理论基础。To investigate the effects of Eimeria tenella heat shock protein 90(EtHSP90) and histone 4(EtH4) on apoptosis in chicken embryo fibroblast cells(DF-1 cells) in vitro,this study constructed eukaryotic fluorescent expression plasmids pEGFP-N1-EtHSP90 and pEGFP-N1-EtH4 and transfected them into DF-1 cells.The transfection efficiency was observed using a fluorescence microscope,protein expression was verified by Western blot,and apoptosis in DF-1 cells was detected by flow cytometry.The results confirmed the successful construction of recombinant plasmids pEGFP-N1-EtHSP90 and pEGFP-N1-EtH4.Flow cytometry revealed that the early apoptosis rate in the pEGFP-N1-EtHSP90 group was significantly lower than that in the Blank group,pEGFP-N1 group,and pEGFP-N1-EtH4 group(P<0.05).The early apoptosis rate in the pEGFP-N1-EtH4 group did not differ significantly from the Blank group and the pEGFP-N1 group(P>0.05).Therefore,EtHSP90 can inhibit early cell apoptosis,while EtH4 neither promotes nor inhibits cell apoptosis.These results provide a theoretical basis for further exploration of the apoptosis mechanisms of these two proteins.

关 键 词：柔嫩艾美耳球虫 热休克蛋白90 组蛋白4 细胞凋亡 

分 类 号：S855.9[农业科学—临床兽医学]