加减葛根芩连汤对感染耐药性大肠杆菌雏鸡的治疗效果  

Therapeutic Effect of Modified Gegen Qinlian Decoction on Chickens Infected with Drug-Resistant Escherichia coli

作　　者：刘占通 王泽仁[2] 苗晋鑫[3] 王丽 陈鹏举 LIU Zhantong;WANG Zeren;MIAO Jinxin;WANG Li;CHEN Pengju(Henan Institute of Agricultural,Animal and Aquatic Products Inspection Technology,Zhengzhou 450008,China;Zhoukou Animal Disease Control Center,Zhoukou 466700,China;Chinese Academy of Medical Sciences,Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Institute of Modern Chinese Veterinary Medicine,Zhengzhou 450002,China)

机构地区：[1]河南省农畜水产品检验技术研究院,河南郑州450008 [2]周口市动物疫病预防控制中心,河南周口466700 [3]河南中医药大学中医药科学院,河南郑州450046 [4]河南省现代中兽医研究院,河南郑州450002

出　　处：《中国兽医杂志》2024年第7期108-116,共9页Chinese Journal of Veterinary Medicine

基　　金：国家重点研发计划项目(2017YFD0501400)。

摘　　要：为了评价加减葛根芩连汤对感染耐药性大肠杆菌雏鸡的治疗效果,本试验首先用耐药性大肠杆菌感染4日龄雏鸡,6 h后分别灌服低(0.125 mL/羽)、中(0.25 mL/羽)和高(0.5 mL/羽)剂量的加减葛根芩连汤、锦心口服液(中药对照组)、恩诺沙星(西药对照组)和生理盐水(感染对照组和空白对照组),连续灌服5 d,计算各组雏鸡存活率和治愈率、采食量和饮水量、体重及其增量,分析血液生理生化指标和免疫器官指数。结果显示,低、中和高剂量组雏鸡24 h存活率分别为85%、80%和65%,极显著高于中药对照组(50%)和西药对照组(50%)(P<0.01);中和高剂量组雏鸡7d存活率和治愈率均达70%,极显著高于感染对照组和西药对照组(P<0.01);各感染组雏鸡每日平均采食量和平均饮水量在感染后明显减少,用药后均恢复并趋于稳定,体重保持稳定增长。血液生理生化指标结果显示,低、中和高剂量组白细胞数目显著低于西药对照组(P<0.05),平均血红蛋白含量显著高于空白对照组(P<0.01、P<0.001、P<0.01),中和高剂量组平均红细胞血红蛋白浓度显著低于空白对照组(P<0.05),高剂量组红细胞分布宽度变异系数显著高于其他各组(P<0.05),低、中和高剂量组肌酐含量极显著低于西药对照组(P<0.01)。免疫器官指数结果显示,低和中剂量组感染雏鸡法氏囊和脾脏指数极显著高于西药对照组(P<0.01),高剂量组胸腺指数显著高于中药对照组(P<0.05)。结果表明,不同剂量的加减葛根芩连汤对耐药性大肠杆菌感染雏鸡均具有较好的治疗效果,且中和高剂量的治疗效果显著优于低剂量,具有替代抗生素的潜力,可为耐药性大肠杆菌病的防治提供理论支撑。To evaluate the therapeutic effect of modified Gegen Qinlian decoction on chickens infected with drug-resistant Escherichia coli,this study infected 4-day-old chicks with drug-resistant Escherichia coli.Six hours later,the chickens were orally administered low(0.125 mL/bird),medium(0.25 mL/bird),and high(0.5 mL/bird) doses of modified Gegen Qinlian decoction,Jin Xin oral liquid(Chinese medicine control group),enrofloxacin(Western medicine control group),and physiological saline(Infection control group and Blank control group) continuously for 5 days.The study calculated the survival rate and cure rate of chickens in each group,as well as their feed intake and water consumption,body weight and weight gain,and analyzed blood physiological and biochemical indicators and immune organ indices.The results showed that the 24-hour survival rates of chickens in the Low-,Medium-,and High-dose groups were 85%,80%,and 65%,respectively,significantly higher than those in the Chinese medicine control group(50%) and Western medicine control group(50%)(P<0.01);the 7-day survival rates and cure rates of chickens in the Medium-and High-dose groups were both 70%,significantly higher than those in the Infection control group and Western medicine control group(P<0.01);the average daily feed intake and water consumption of chickens in each infection group decreased significantly after infection,but recovered and stabilized after medication,with stable weight gain.Blood physiological and biochemical indicators showed that the white blood cell count in the Low-,Medium-,and High-dose groups was significantly lower than that in the Western medicine control group(P<0.05),and the average hemoglobin content was significantly higher than that in the Blank control group(P<0.01,P<0.001,P<0.01);the average red blood cell hemoglobin concentration in the Medium-and High-dose groups was significantly lower than that in the Blank control group(P<0.05),and the red blood cell distribution width coefficient of variation in the High dose group was signif

关 键 词：加减葛根芩连汤 耐药性大肠杆菌 治疗效果 

分 类 号：S852.65[农业科学—基础兽医学] R28[农业科学—兽医学]