廉江高桥红树林沉积物微生物群落结构特征及其与营养因子相关性  

Microbial Community Structure in Mangrove Sediments of Lianjiang Gaoqiao and Its Correlation with Nutrient Factors

作　　者：梁文雅 王冼民 刘琦 LIANG Wenya;WANG Xianmin;LIU Qi(Faculty of Chemistry and Environmental Science,Guangdong Ocean University,Zhanjiang 524088,China;School of Geographical Sciences,Lingnan Normal University,Zhanjiang 524048,China)

机构地区：[1]广东海洋大学化学与环境学院,广东湛江524088 [2]岭南师范学院地理科学学院,广东湛江524048

出　　处：《广东海洋大学学报》2024年第4期80-87,共8页Journal of Guangdong Ocean University

摘　　要：【目的】研究廉江高桥红树林沉积物中微生物群落构特征,及其与环境营养因子的相关性,为了解该研究区域红树林生态系统功能和红树林湿地保护提供基础。【方法】利用16S rDNA高通量测序手段,分析红树林生态系统表层沉积物中微生物的组成、多样性和群落结构,并通过相关性分析探究其对环境营养因子的响应。【结果】研究区域沉积物中微生物优势细菌类群为变形菌门(Proteobacteria),绿弯菌门(Chloroflexi),酸杆菌门(Acidobacteria),拟杆菌门(Bacteroidetes)和芽单胞菌门(Gemmatimonadetes),其中变形菌门是相对丰度最高的类群,占比46.6%~68.9%。微生物α多样性中Shannon指数变化范围为9.95~10.34,Chao1指数变化范围为1 990~2 527,这两个指数在中高潮带间均存在显著差异(P <0.05)。沉积物中总磷、有效磷、总氮和氨氮的质量分数变化范围分别为383.575~760.283、41.772~97.076、820.201~1 452.442、1.511~9.048 mg·kg^(-1),且总磷在中高潮带间存在显著差异(P <0.05)。沉积物中营养因子对门水平上微生物群落组成的解释量为46%,其中总有机碳(TOC)和总氮是对群落结构影响较大的营养因子。【结论】廉江高桥红树林沉积物中微生物多样性丰富,中潮带微生物多样性和丰富度显著高于高潮带,且中潮带采样点的样本与高潮带的分离程度较好;不同分类的微生物对环境中营养因子具有不同程度的响应,TOC是影响中潮带沉积物中微生物群落组成和结构的关键因子,而氮磷是控制高潮带微生物群落结构的主要营养因子。【Objective】 To study the microbial community structure in mangrove sediments of Lianjiang Gaoqiao and its correlation with environmental nutrition factors.It provides the basis for understanding the mangrove ecosystem function and mangrove wetland protection in this study area.【Method】 The composition,diversity and community structure of microorganisms in the surface sediments of mangrove ecosystems were studied by 16S rDNA high throughput sequencing,and their responses to environmental nutrient factors were investigated by correlation analysis.【Result】 The dominant bacterial groups in the sediments of the study area were Proteobacteria,Chloroflexi,Acidobacteria,Bacteroidetes and Gemmatimonadetes.Proteobacteria was the most abundant group,accounting for 46.6% ― 68.9%.In microbial α diversity,the Shannon index ranges from 9.95 to 10.34,and the Chao1 index ranges from 1 990 to 2 527.There were significant differences between the middle tidal zone and the high tidal zone(P < 0.05).The mass fractions of TP,AP,TN and NH_(4)^(+) in sediments ranged from 383.575-760.283,41.772-97.076,820.201-1 452.442,1.511-9.048 mg · kg^(-1) respectively.There was significant difference in TP between middle high water zones(P < 0.05).The nutrient factors in sediments explained 46% of the microbial community composition at the gate level,among which Total organic carbon(TOC) and total nitrogen were the nutrient factors that had great influence on the community structure.【Conclusion】 Microbial diversity was abundant in mangrove sediments in Lianjiang Gaoqiao.Compared with the high tidal zone,the microbial diversity and richness in the middle tidal zone were significantly higher than those in the high tidal zone,and the sampling points in the middle tidal zone were better separated from the high tidal zone.Different types of microorganisms had different degrees of response to environmental nutrient factors.TOC was the key factor affecting the composition and structure of microbial community in sediments in the middle t

关 键 词：红树林沉积物 微生物群落 16S rDNA高通量测序 多样性 相关性 

分 类 号：Q938[生物学—微生物学]