静电纺丝制备高度取向胶原纤维及其性能研究  

Fabrication and Properties of Highly Aligned Electrospun Collagen Fibers

作　　者：杨超 邹杰 李鉴墨 YANG Chao;ZOU Jie;LI Jian-mo(Konee Biomedical(Shenzhen)Co.,Ltd.,Shenzhen 518118,China)

机构地区：[1]康膝生物医疗(深圳)有限公司,深圳518118

出　　处：《高分子通报》2024年第7期956-966,共11页Polymer Bulletin

基　　金：深圳市科技计划项目(项目号KQTD20190929172501638)。

摘　　要：本文以静电纺丝工艺制备了电纺胶原蛋白纤维(ECF),系统研究了纺丝液组成、电纺条件以及环境因素对ECF的影响。扫描电镜用于ECF的形貌分析。结果表明在较宽松的电纺条件:质量浓度为70%~80%的乙酸水溶液作为溶剂,胶原质量浓度为15%~18%,纺丝液推注速度为0.15~0.3mL/h,纺丝电压为16~24 kV,纺丝间距8~12 cm,以22G或21G钝针作为喷丝口,纺丝室的相对湿度50%~55%,温度30~32℃,都能得到表面形貌均匀的ECF。进一步以盘状定向纤维收集器制备了取向电纺胶原纤维(AECF),纤维夹角和Herman取向因子用于表征纤维取向,发现AECF取向随着收集器转速增加而增加。圆二色谱用于表征胶原电纺前后是否具有三螺旋结构,透射电镜用于观察AECF自组装能力,噻唑蓝(MTT)法用于评价AECF的细胞毒性。结果表明AECF具有天然胶原一样的三螺旋结构,具备自组装能力,无细胞毒性,可用于组织工程支架。Electrospun collagen fibers(ECF)are fabricated,and the effects of electrospun solution composition,electrospun conditions and environmental factors on electrospun collagen fibers are systematically studied in this article.Scanning electron microscopy is used to analyze the morphology of ECF.The results show that ECF with uniform surface morphology can be obtained under a wide range of electrospun conditions:70%-80%weight concentration acetic acid solution as solvent,collagen weight concentration as 15%-18%,injection speed of electrospun solution as 0.15-0.3 mL/h,electrospun voltage as 16-24 kV,spinning gap as 8-12 cm,22G or 21G blunt needle as spinning nozzle,the relative humidity as 50%-55%,and temperature at 30-32℃of electrospun chamber.Based on the above conditions,aligned electrospun collagen fibers(AECF)are prepared by the disc-like directional fiber collector.Average angle between fibers and Herman orientation factor are used to characterize fiber orientation,and it is found that AECF orientation increased with collector speed.The triple helix structure and self-assembly capacity of collagen in AECF are characterized by circular dichroism and transmission electron microscopy respectively,while the cytotoxicity of AECF is evaluated by Thiazolgl Blue(MTT).The results show that collagen in AECF has a natural collagen-like triple helix structure,self-assembly ability,no cytotoxicity,making it suitable for tissue engineering scaffolds.

关 键 词：胶原 静电纺丝 取向 自组装 圆二色谱 细胞毒性 

分 类 号：TQ340.64[化学工程—化纤工业]