Engineering self-catabolic DNAzyme nanospheres for synergistic anticancer therapy  

作　　者：Yu Chen Yu Guo Jiaoli Wang Ruiting Liu Xiaohai Yang Kemin Wang Ying Pu Hui Shi Jin Huang 

机构地区：[1]Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province,Hunan University,Changsha 410082,China [2]National Clinical Research Center for Geriatric Disorders,Department of Geriatrics,Xiangya Hospital,Central South University,Changsha 410008,China

出　　处：《Science China Chemistry》2024年第7期2412-2422,共11页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(22174042 and 22374038);the Natural Science Foundation for Distinguished Young Scholars of Hunan Province(2021JJ10011);the Postgraduate Scientific Research Innovation Project of Hunan Province(CX20220390)。

摘　　要：DNAzyme-based gene therapy faces some challenges including cell penetration,activity limitation,and co-delivery functions.Self-assembled DNA nanomedicine has attracted widespread attention due to its many advantages.It is urgent to develop a universal DNA degradation strategy for precise programmable drug release.Herein,we reported a self-catabolic DNAzyme nanospheres(SCNS),which could simultaneously achieve cell penetration,activity enhancement,and co-delivery functions.The SCNS were assembled through Y-DNA stepwise hybridization with each other,which were then loaded with aptamer(Apt),doxorubicin(Dox),and zinc oxide nanoparticles(ZnO NPs).The acid-triggered dissociation of ZnO NPs leads to the generation of Zn^(2+)ions cofactors for immediately self-catabolic DNAzyme nanospheres.After the disassembly of the SCNS,three types of anticancer treatments would be activated,which include Zn^(2+)involved reactive oxygen species(ROS),Dox-induced chemotherapy,and DNAzyme-based gene therapy.The experimental results show that the nanoplatform(Apt-SCNS-Dox-ZnO)has a good tumor-killing effect and minimal side effects.As a smart self-driven drug delivery nanoplatform,it is anticipated to displace extraordinary potential in biomedicine and bioengineering.

关 键 词：DNAzyme nanosphere ZnO NPs ROS DOX anticancer therapy 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R943[医药卫生—药剂学]