Hi-TOM 2.0:an improved platform for high-throughput mutation detection  

作　　者：Tingting Sun Qing Liu Xi Chen Fengyue Hu Kejian Wang 

机构地区：[1]State Key Laboratory of Rice Biology and Breeding,China National Rice Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310006,China [2]Hainan Seed Industry Laboratory,Sanya 572025,China

出　　处：《Science China(Life Sciences)》2024年第7期1532-1534,共3页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(32025028,U20A2030);Zhejiang Provincial Key Research and Development Program(2021C02063-6);Earmarked Fund(CARS--01);the Hainan Seed Industry Laboratory(B23CJ0208);Zhejiang Provincial Natural Science Foundation of China(LTGN24C130005)。

摘　　要：Dear Editor,CRISPR/Cas systems,which can create novel mutations by manipulating the genome or transcriptome,have been developed as a powerful molecular tool for gene editing in various organisms(Cheng et al.,2022;Gao,2021).However,identifying mutations in large samples and decoding chimeric mutations are time-consuming,labor-intensive.

关 键 词：CRISPR/Cas consuming MUTATION 

分 类 号：Q78[生物学—分子生物学]