共刺激分子B7-H6在结直肠癌中的表达及其对结直肠癌细胞增殖侵袭的促进作用  

Expression of Costimulatory Molecule B7-H6 in Colorectal Cancer Tissues and its Effect on the Proliferation and Invasion of Colorectal Cancer Cells

作　　者：汤权 王进[3] 赵鑫[1] 虞竹雯 TANG Quan;WANG Jin;ZHAO Xin;YU Zhu-wen(Department of General Surgery,First Affiliated Hospital of Soochow University,Suzhou,Jiangsu,215006,China;Depaartment of General Surgery,Suzhou Municipal Hospital,Suzhou,Jiangsu,215008,China;Department of General Surgery,Dushuhu Hospital Affiliated to Soochow University,Suzhou,Jiangsu,215123,China;Department of Gastroenterology,First Affiliated Hospital of Soochow University,Suzhou,Jiangsu,215006,China)

机构地区：[1]苏州大学附属第一医院普外科,江苏苏州215006 [2]苏州市立医院普外科,江苏苏州215008 [3]苏州大学附属独墅湖医院普外科,江苏苏州215123 [4]苏州大学附属第一医院消化内科,江苏苏州215006

出　　处：《中国血液流变学杂志》2024年第1期9-18,F0002,共11页Chinese Journal of Hemorheology

基　　金：国家自然科学基金资助项目(82073180);江苏省青年医学人才(QNRC2016732);苏州市“姑苏卫生人才”计划青年拔尖人才(2018-057);姑苏卫生人才培养项目(GSWS2019028);苏州市“科教兴卫”青年科技项目(KJXW2021086)。

摘　　要：目的研究结直肠癌组织中共刺激分子B7-H6的表达与临床病理特征之间的关系;研究共刺激分子B7-H6对结直肠癌细胞增殖和侵袭能力的影响。方法免疫组化和RT-qPCR分别检测癌组织和正常癌旁组织中B7-H6的蛋白和mRNA表达水平。统计分析B7-H6的表达水平与结直肠癌患者的肿瘤大小、分化程度、TNM分期和淋巴结转移等临床病理特征之间的相关性。构建shRNA慢病毒载体,建立低表达B7-H6的人结肠癌细胞株。免疫荧光验证慢病毒转染效率。流式细胞术和RT-qPCR分别检测转染前后B7-H6蛋白及mRNA的表达水平。MTT实验检测结直肠癌细胞的增殖能力。Transwell实验检测结直肠癌细胞的侵袭能力。结果结直肠癌组织中B7-H6蛋白的阳性表达率明显高于正常癌旁组织(72.5%vs 22.5%,P<0.001)。结直肠癌组织中B7-H6 mRNA的表达水平明显高于正常癌旁组织(P<0.001)。结直肠癌组织中B7-H6的阳性表达与结直肠癌患者肿瘤分化程度、TNM分期和淋巴结转移有关(P<0.05),而与年龄、性别、肿瘤大小无明显相关性(P>0.05)。shRNA慢病毒载体转染可以成功构建稳定低表达B7-H6的结直肠癌细胞株。敲低组(SW620-sh-B7-H6、LOVO-sh-B7-H6)相较于阴性对照组(SW620-NC、LOVO-NC),肿瘤细胞增殖和侵袭的能力明显减弱(P<0.001)。结论B7-H6在结直肠癌组织中异常高表达与结直肠癌患者的TNM分期、分化程度和淋巴结转移情况有关;敲低B7-H6可以抑制结直肠癌细胞的增殖与侵袭。Objective To investigate the relationship between the expression of costimulatory molecule B7-H6 in colorectal cancer tissues and clinicopathological features,and its effect on the proliferation and invasion of colorectal cancer cells.Methods Immunohistochemistry and RT-qPCR were used to detect the expression of B7-H6 protein and mRNA in cancer tissues and normal adjacent tissues.The correlation between the expression level of B7-H6 and various clinical characteristics of colorectal cancer patients such as tumor size,differentiation degree,TNM stage and lymph node metastasis was analyzed.shRNA lentiviral vector was constructed to establish a human colon cancer cell line with low expression of B7-H6.Immunofluorescence was used to verify the transfection efficiency of lentivirus.Flow cytometry and RT-qPCR were used to detect the expression level of B7-H6 protein and mRNA before and after transfection,respectively.MTT assay was used to detect the proliferation ability of colorectal cancer cells.The invasive ability of colorectal cancer cells was detected by Transwell assay.Results Compared with adjacent tissues,the expression level of B7-H6 protein in colorectal cancer tissues was significantly increased(72.5%vs 22.5%,P<0.001).Compared with adjacent tissues,the expression of B7-H6 mRNA in colorectal cancer tissues was significantly increased(P<0.001).The positive expression of B7-H6 was related to tumor differentiation,TNM stage and lymph node metastasis in patients with colorectal cancer(P<0.05),but not with age,gender and tumor size(P>0.05).shRNA lentiviral vector transfection can successfully construct a stable colorectal cancer cell line with low expression of B7-H6.Compared with the negative control group(SW620-NC,LOVO-NC),the cell proliferation and invasion ability of knockdown group(SW620-sh-B7-H6,LOVO-sh-B7-H6)were significantly decreased(P<0.001).Conclusion The abnormal high expression of B7-H6 in colorectal cancer tissues is related to the degree of differentiation,TNM stage and lymph node metastasis of co

关 键 词：B7-H6 结直肠癌 临床病理特征 增殖 侵袭 

分 类 号：R446.11[医药卫生—诊断学] R587.1[医药卫生—临床医学]