基于Nrf2/HO-1通路探讨针灸预处理对急性胃黏膜损伤大鼠的影响及机制  

Analysis of Effect and Mechanism of Acupuncture and Moxibustion Preconditioning on Rats with Acute Gastric Mucosal Injury Based on Nrf2/HO-1 Pathway

作　　者：李盼 兰永利 韦芳 阿斯卡尔·牙生 霍新慧[1] LI Pan;LAN Yongli;WEI Fang;Askar Yasheng;HUO Xinhui(College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830001,China)

机构地区：[1]新疆医科大学中医学院,新疆维吾尔自治区乌鲁木齐830001

出　　处：《山东中医药大学学报》2024年第4期453-458,477,共7页Journal of Shandong University of Traditional Chinese Medicine

基　　金：新疆维吾尔自治区自然科学基金项目(编号:2021D01C224);新疆维吾尔自治区天山英才计划第三期项目(批文号:新人社函[2021]37号)。

摘　　要：目的:观察针灸预处理对急性胃黏膜损伤大鼠的影响,并探讨其机制。方法:将52只SPF级大鼠随机分为正常组、模型组、灸预组和针预组,每组13只,先分别对灸预组和针预组大鼠施以艾灸和针刺中脘、足三里,每次20 min,每日1次,连续7 d;然后对模型组、灸预组和针预组采用无水乙醇与阿司匹林混悬液灌胃制备大鼠急性胃黏膜损伤模型。比较各组大鼠胃黏膜损伤指数(UI)和病理学变化,酶联免疫吸附测定(ELISA)法检测各组大鼠胃黏膜组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)含量和肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平,免疫组织化学法检测核因子E2相关因子2(Nrf2)和血红素氧合酶-1(HO-1)蛋白表达。结果:与正常组相比,模型组大鼠UI升高(P<0.01),胃黏膜组织出现充血和糜烂等病理表现,提示模型制备成功,血清SOD、GPX含量下降(P<0.01),血清MDA、TNF-α、IL-6水平及胃黏膜组织Nrf2、HO-1蛋白表达水平上升(P<0.01)。与模型组相比,灸预组和针预组UI显著降低(P<0.01);灸预组大鼠胃黏膜未见明显病理改变,针预组大鼠胃黏膜大部分完整,只有少量红细胞渗出。与模型组相比,灸预组和针预组血清SOD、GPX含量上升(P<0.01),血清MDA、TNF-α、IL-6水平及胃黏膜组织Nrf2、HO-1蛋白表达水平下降(P<0.05或P<0.01)。与针预组相比,灸预组血清SOD、GPX含量上升(P<0.01),血清MDA、TNF-α、IL-6水平及胃黏膜组织Nrf2、HO-1蛋白表达水平下降(P<0.05或P<0.01)。结论:针灸预处理可以预防大鼠急性胃黏膜病理损伤,其作用机制可能与调控Nrf2/HO-1通路、缓解氧化应激反应和减轻炎症反应有关。Objective:To observe the effect of acu-puncture and moxibustion preconditioning on rats with acute gastric mucosal injury,and explore its mechanism.Methods:A total of 52 SPF rats were randomly divided into normal group,model group,moxibustion preconditioning group and acupuncture precon-ditioning group,with 13 rats in each group.Rats in the moxibustion preconditioning group and acupuncture preconditioning group were preconditioned with moxibustion and acupuncture at Zhongwan(RN12)and Zusanli(ST36),20 minutes each time,once a day,for 7 consecutive days.Then,rats in the model group,the moxi-bustion preconditioning group and acupuncture preconditioning group were given anhydrous alcohol with aspirin suspension to prepare the model rats with acute gastric mucosal injury.Ulcer index(UI)and pathological changes were compared in each group.Enzyme linked immunosorbent assay(ELISA)was used to detect the content of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GPX),and the levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in gastric mucosa of each group.The expressions of nuclear factor erythroid 2 related factor 2(Nrf2)and heme oxygenase-1(HO-1)protein were detected by immunohistochemistry.Results:Compared with the normal group,the UI in the model group was increased(P<0.01),and pathological changes such as congestion and erosion were found in the gastric mucosa of rats in the model group,suggesting that the model was successfully established.The content of serum SOD and GPX decreased(P<0.01),while the levels of serum MDA,TNF-αand IL-6,and protein expression of Nrf2 and HO-1 in gastric mucosa increased(P<0.01).Compared with the model group,the UI in the moxibustion preconditioning group and acupuncture preconditioning group were significantly lower(P<0.01).In the moxibustion preconditioning group,no obvious pathological changes were found in the gastric mucosa,while in the acupuncture preconditioning group,the gastric mucosa was mostly intact with only a small amount of red blood

关 键 词：针灸预处理 胃黏膜损伤 治未病 氧化应激 炎症反应 核因子E2相关因子2 血红素氧合酶-1 大鼠 

分 类 号：R246.1[医药卫生—针灸推拿学]