基于TLR2/MyD88信号通路探究五味子乙素对全身炎症反应综合征的影响  

Effect of Schisandrae B on SIRS Based on TLR2/MyD88 Signaling Pathway

作　　者：许亚威 赵紫涵 李贺[1] 王春梅[1] 孙靖辉[1] 张志宏 陈曦[2] 张成义[1] XU Yawei;ZHAO Zihan;LI He;WANG Chunmei;SUN Jinghui;ZHANG Zhihong;CHEN Xi;ZHANG Chengyi(Pharmacy College of Beihua University,Jilin 132013,China;Basic Medical College of Beihua University,Jilin 132013,China)

机构地区：[1]北华大学药学院,吉林吉林132013 [2]北华大学基础医学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2024年第5期603-608,共6页Journal of Beihua University（Natural Science）

基　　金：北华大学研究生创新计划项目(2022066)。

摘　　要：目的观察五味子乙素(Schisandrin B,SchB)通过TLR2/MyD88信号通路对酵母聚糖诱导的小鼠全身炎症反应综合征(Systemic inflammatory response syndrome,SIRS)的影响。方法采用腹腔注射酵母聚糖方法造模,记录小鼠体温及白细胞变化,应用酶联免疫吸附法检测血清中TNF-α、IL-1β及IL-6水平,应用试剂盒检测ALT、AST、Cr、BUN含量;HE染色观察各组织病理学变化;应用Western Blot法检测组织中TLR2、MyD88、NF-κB、HMGB1蛋白表达水平。结果SchB使小鼠体温升高显著(P<0.01),白细胞数量增多明显(P<0.01);SchB能显著降低TNF-α、IL-1β、IL-6、ALT、AST、Cr、BUN水平(P<0.001);Western Blot结果显示,SchB能显著降低组织中TLR2、MyD88、NF-κB、HMGB1的表达水平(P<0.01)。结论SchB能够减轻由酵母聚糖导致的SIRS,其作用机制主要是通过调控TLR2/MyD88信号通路缓解炎症。Objective To investigate the effect of Schisandrin B on zymosan-induced SIRS in mice through TLR2/MyD88 signaling pathway.Method The model was established by intraperitoneal injection of zymosan.The changes of body temperature and white blood cells were recorded.The levels of TNF-α,IL-1βand IL-6 in serum were detected by enzyme-linked immunosorbent assay.ALT,AST,Cr,BUN in serum were detected with the kits;HE staining was used to observe the pathological changes of each tissue.Western Blot was used to detect the expression levels of TLR2,MyD88,NF-κB,HMGB1 in tissues.Results SchB can make temperature in mice increase significantly(P<0.01),blood cell in mice increase significantly(P<0.01).SchB could significantly reduce the levels of TNF-α,IL-1β,IL-6,ALT,AST,Cr and BUN(P<0.001).Western Blot results showed that SchB could significantly reduce the expression levels of TLR2,MyD88,NF-κB and HMGB1 in tissues(P<0.01).Conclusion SchB can alleviate the SIRS induced by zymosan,and the mechanism is mainly to regulate TLR2/MyD88 signaling pathway to alleviate inflammation.

关 键 词：五味子乙素 全身炎症反应综合征 酵母聚糖 TLR2/MyD88信号通路 

分 类 号：R285.5[医药卫生—中药学]