桑白皮总黄酮对小鼠肺癌模型的影响及相关机制  

Effect and Related Mechanisms of Total Flavonoids from Mulberry Bark on Mouse Lung Cancer Model

作　　者：郭楠楠[1] 苏翔宇[1] 杜海娜 任安晶 GUO Nanna;SU Xiangyu;DU Haina;REN Anjing(Zhongda Hospital of Southeast University,Nanjing 211800,China;Nanjing Hospital of Traditional Chinese Medicine,Nanjing 210022,China;The First Affiliated Hospital of Nanjing Medical University,Nanjing 210006,China)

机构地区：[1]东南大学附属中大医院,江苏南京211800 [2]南京市中医院,江苏南京210022 [3]南京医科大学第一附属医院,江苏南京210006

出　　处：《北华大学学报（自然科学版）》2024年第4期454-459,共6页Journal of Beihua University（Natural Science）

基　　金：江苏省老年健康基金项目(LK2021022);江苏省血地寄防科研项目(X202104)。

摘　　要：目的 探讨桑白皮总黄酮对小鼠肺癌模型的影响及相关机制。方法 将小鼠分为空白对照组(20只),模型组(14只),桑白皮总黄酮低(16只)、中(16只)、高剂量组(18只)。使用乌拉坦诱导建立小鼠肺癌模型,从造模首日开始,桑白皮总黄酮低、中、高剂量组分别灌胃桑白皮总黄酮15、30、60 mg/kg,对照组、模型组灌胃给予等体积的蒸馏水,1次/d,连续16周,第16周试验结束时统计各组存活小鼠的肺癌发生率、肺肿瘤结节数量。酶联免疫吸附法检测血清鳞状上皮细胞癌抗原、癌胚抗原、神经特异性烯醇化酶(NSE)、糖类抗原125(CA125)、糖类抗原199(CA199)浓度;免疫组化法检测小鼠肺组织中N-钙黏蛋白、E-钙黏蛋白表达阳性率;Western blot检测小鼠肺组织中PI3K、AKT、p-AKT蛋白表达水平。结果 肺癌发生率、肺肿瘤结节数量在各组间比较差异具有统计学意义(P<0.01),各剂量桑白皮总黄酮组肺癌发生率、肺肿瘤结节数量均明显低于模型组(P<0.05);随着桑白皮总黄酮剂量的增加,肺癌发生率、肺肿瘤结节数量下降幅度逐渐明显。模型组血清鳞状上皮细胞癌抗原、癌胚抗原、NSE、CA125、CA199明显高于空白对照组(P<0.01),各剂量桑白皮总黄酮组血清鳞状上皮细胞癌抗原、癌胚抗原、NSE、CA125、CA199明显低于模型组(P<0.01);随着桑白皮总黄酮剂量的增加,血清鳞状上皮细胞癌抗原、癌胚抗原、NSE、CA125、CA199浓度逐渐降低。模型组小鼠肺组织中N-钙黏蛋白表达阳性率明显高于空白对照组,E-钙黏蛋白表达阳性率明显低于空白对照组(P<0.01),与模型组比较,各剂量桑白皮总黄酮组小鼠肺组织中N-钙黏蛋白表达阳性率明显降低,E-钙黏蛋白表达阳性率明显升高(P<0.05);随着桑白皮总黄酮剂量的增加,上述变化逐渐明显。模型组小鼠肺组织中PI3K、AKT、p-AKT蛋白表达水平明显高于空白对照组(P<0.01),与模型组比较,各剂量�Objective To explore the effect and related mechanisms of total flavonoids from mulberry bark on mouse lung cancer model.Method A mouse lung cancer model was established by inducing with Ulatan.Starting from the first day of modeling,the low-dose group,medium dose group and high-dose group of total flavonoids in mulberry bark were given 15 mg/kg,30 mg/kg and 60 mg/kg of total flavonoids in mulberry bark by gavage,respectively.The control group and model group were given equal volumes of distilled water by gavage once a day for 16 consecutive weeks.Serum levels of squamous cell carcinoma antigen,carcinoembryonic antigen,neuron specific enolase(NSE),carbohydrate antigen 125(CA125),and carbohydrate antigen 199(CA199)were measured by ELISA.The positive expression rates of N-cadherin and E-cadherin in lung tissue were detected by using immunohistochemical method;Western blot was used to detect the protein expression of PI3K,AKT and p-AKT in mouse lung tissue.Results The incidence of lung cancer and the number of lung tumor nodules showed statistically significant among the groups(P<0.01);The incidence of lung cancer and the number of lung tumor nodules treated with different doses of total flavonoids from mulberry bark were significantly lower than those in the model group(P<0.05).As the dose of total flavonoids from mulberry bark increased,the incidence of lung cancer and the number of lung tumor nodules gradually decreased.The serum levels of squamous cell carcinoma antigen,carcinoembryonic antigen,NSE,CA125,and CA199 in the model group were significantly higher than those in the blank control group(P<0.01);The serum levels of squamous epithelial cell carcinoma antigen,carcinoembryonic antigen,NSE,CA125,and CA199 in the total flavonoid group at different doses were significantly lower than those in the model group(P<0.01).As the total flavonoid dose of mulberry bark increased,the serum concentrations of squamous epithelial cell carcinoma antigen,carcinoembryonic antigen,NSE,CA125,and CA199 gradually decreased.The po

关 键 词：肺癌 小鼠 桑白皮总黄酮 上皮-间质转化 PI3K/AKT信号通路 

分 类 号：R282[医药卫生—中药学] R734.2[医药卫生—中医学]