废弃矿井环境中微生物多样性与代谢功能预测  

Microbial Diversity and Metabolic Function Prediction in Abandoned Mine Environment

作　　者：杨启林 杨爱江[1,2] 李彦澄 张玉多 廖珣 YANG Qi-lin;YANG Ai-jiang;LI Yan-cheng;ZHANG Yu-duo;LIAO Xun(Coll.of Res.&Environ.Engin.,Guizhou Uni.,Key Lab.of Karst Geol.Res.&Environ.Minist.of Educ.,Guiyang 550025;Guizhou Karst Environ.&Ecosystem Minist.of Educ.Field Sci.Observ.&Res.Station,Guiyang 550025)

机构地区：[1]贵州大学资源与环境工程学院喀斯特地质资源与环境教育部重点实验室,贵州贵阳550025 [2]贵州喀斯特环境生态系统教育部野外科学观测研究站,贵州贵阳550025

出　　处：《微生物学杂志》2024年第3期40-48,共9页Journal of Microbiology

基　　金：贵州省科技计划项目(黔科合支撑[2021]一般475)。

摘　　要：矿山废水已成为主要的环境污染问题之一,为探究废弃矿井排水生态环境中的微生物群落多样性和功能,选取贵州省遵义市某废弃煤矿,采用16S rRNA基因高通量测序技术研究矿井巷道表层沉积物的细菌和古菌群落组成。结果表明,所有样品中细菌多样性和丰度均高于古菌,细菌群落由52个门820个属组成,其优势菌属主要为A4b科中属水平的未定细菌、SBR1031目中属水平的未定细菌和KD4-96纲中属水平的未定细菌;古菌群落由12个门58个属组成,优势菌门为泉古菌门(Crenarchaeota),优势菌属主要为暂定属Candidatus_Nitrocosmicus和暂定属Candidatus_Nitrsosphaera。PICRUSt2功能预测表明,细菌中包含3条硫代谢循环途径,分别为同化硫酸盐还原、异化硫酸盐氧化/还原和SOX络合物氧化硫代硫酸盐,古菌中包含2条硫代谢循环途径,分别为同化硫酸盐还原和SOX络合物氧化硫代硫酸盐,共检测到18个细菌硫代谢基因和13个古菌硫代谢基因。关于废弃矿井环境中的微生物及其代谢功能分析,能进一步拓展对矿山环境微生物系统的认识。As one of the main environmental pollutions,mine wastewater in this study,sampled from an abandoned coal mine in Zunyi City,Guizhou Province,was assayed with 16S rRNA gene sequencing technology,to reveal its bacterial and archaeal community and composition of the surface sediments along the mine galleries.The results showed that bacterial diversity and abundance in all samples were higher than that of archaea,the bacterial community consisted of 52 phyla and 820 genera.The dominant bacterial were genera level of the family of norank_f_A4b,and genera level of the order of norank_f_SBR1031,and genera level of the class of norank_f_KD4-96.Archaeal community consisted of 12 families and 58 genera,with the dominant bacterial phylum being Crenarchaeota,and dominant bacterial genera provisionally as Candidatus_Nitrocosmicus and provisionally as Candidatus_Nitrsosphaera.PICRUSt2 function prediction suggested that bacteria and archaea exhibited three sulfur metabolism cycle pathways respectively were assimilatory sulfate reduction and thiosulfate oxidation of SOX complex,and archabacteria included two sulfur metabolism pathways,respectively were assimilatory sulfate reduction thiosulfate oxidation of SOX complex,with a total of 18 bacterial sulfur metabolism genes and 13 archaeal sulfur metabolism genes were determined.In this study,the analyses of microorganism and its metabolic function in abandoned mine environment can further expand the understanding of mine environmental microbial system.

关 键 词：矿山环境 细菌与古菌群落 多样性 功能预测 硫代谢循环 

分 类 号：Q939.99[生物学—微生物学]