黄原胶寡糖的制备、分离及表征研究进展  

Advances in Preparation,Separation and Characterization of Xanthan Gum Oligosaccharide

作　　者：王倩 俞志敏[1] 杨帆[1] 李宪臻[1] WANG Qian;YU Zhi-min;YANG Fan;LI Xian-zhen(Coll.of Bio-Engin.,Dalian Engin.Uni.,Dalian 116034;Dalian Inst.of Chem.Physics,CAS.,Dalian 116023)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]中国科学院大连化学物理研究所生物技术研究部,辽宁大连116023

出　　处：《微生物学杂志》2024年第3期96-112,共17页Journal of Microbiology

基　　金：国家自然科学基金项目(32072160,31671796)。

摘　　要：黄原胶寡糖是通过降解黄原胶得到的由2~20个单糖通过糖苷键结合形成的低分子聚合物。黄原胶寡糖理化性质稳定,相比于黄原胶具有易溶于水、黏度低、生物利用度高等特点,具有抗菌、抗氧化、抗肿瘤活性,对植物具有诱导抗病性,能够调节植物生长等广泛的生物活性,具有很好的应用前景,成为近年来寡糖领域的研究热点。黄原胶寡糖的活性与其结构特征密切相关,本文对黄原胶寡糖制备、分离纯化及结构表征进行综述,重点讨论了不同模式下的色谱分离技术及质谱和核磁共振波谱结构表征方法,阐述了各种方法的原理和应用范围,并对其优越性和局限性进行了总结,以期为黄原胶寡糖的进一步研究和应用提供参考。Xanthan gum oligosaccharide(XGOS)is a type of low molecular weight polymer composed of 2-20 monosaccharides,which are covalently linked through glycosidic bonds.In comparison to xanthan,XGOS,which has low viscosity,good water solubility,high chemical stability,and good bioavailability,has become a research hotspot in the field of oligosaccharide due to its many biological activities such as antitumor,antioxidant,antibacterial,inducing plant resistance and promoting plant growth.The activity of xanthan gum oligosaccharides is closely related to their structural characteristics.In this paper,the preparation,separation,and purification of xanthan gum oligosaccharides and their structural characterization are reviewed,with emphasis on chromatographic separation techniques in different modes and structural characterization methods by mass spectrometry and nuclear magnetic resonance spectroscopy.The principles and application scope of the various methods are summarized,and their superiority and limitations are analyzed.The information presented here is intended to serve as a useful reference for future research and practical applications of xanthan gum oligosaccharides.

关 键 词：黄原胶 寡糖 制备 分离纯化 结构表征 

分 类 号：Q936[生物学—微生物学]