苹果TIPs亚家族成员鉴定与干旱胁迫响应分析  

Identification and expression of the TIP subfamily in apple in response to drought stress

作　　者：刘河 卢世雄 梁国平 毛娟[1] 陈佰鸿[1] LIU He;LU Shixiong;LIANG Guoping;MAO Juan;CHEN Baihong(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学园艺学院,兰州730070

出　　处：《西北植物学报》2024年第7期1083-1093,共11页Acta Botanica Boreali-Occidentalia Sinica

基　　金：甘肃省科技重大专项(22ZD6NA045);甘肃省2023年现代丝路寒旱农业科技项目(KJZC-2023-13)。

摘　　要：【目的】鉴定并分析苹果液泡膜内在蛋白(TIP)亚家族成员,探究该亚家族成员在苹果干旱胁迫下的表达模式,为研究苹果抗旱性基因资源挖掘和利用提供参考。【方法】通过生物信息学方法对苹果MdTIPs全基因组进行鉴定,分析其家族成员的理化性质、基因结构、保守基序、顺式作用元件、系统进化树等,并结合实时荧光定量PCR(qRT-PCR)分析其在干旱胁迫下不同器官中的表达模式。【结果】在苹果基因组中,共检索到13个MdTIP基因,大部分成员亚细胞定位预测在质膜上,分布于10条染色体上,且每条染色体定位有1~3个成员;该基因家族成员的启动子区域包含多种响应激素和逆境胁迫的应答元件;qRT-PCR显示,MdTIPs亚家族成员在根中除MdTIP1;1外其余均受上调表达,且MdTIP1;3和MdTIP1;4与对照相比,分别上调表达了5.27倍和5.69倍,表明其是参与调控干旱胁迫的关键基因。【结论】鉴定并提供了MdTIPs亚家族成员信息,10个MdTIPs亚家族成员在根、茎、叶中差异表达,12个亚家族成员在根中高表达。[Objective]This study aims to identify and analyze the members of the tonoplast intrinsic protein(TIP)subfamily in apple,and to investigate the expression patterns under drought stress.It provides information for further research on utilization of drought resistance gene resources in apple.[Methods]The MdTIPs in apple genome was identified by bioinformatics methods.The physicochemical properties,gene structures,conserved motifs,cis-regulatory elements,and phylogenetic trees,etc.of the subfamily members were analyzed.The expression patterns of MdTIPs in different organs under drought stress were analyzed by qRT-PCR.[Results]A total of 13 MdTIP genes were identified in the apple genome,and most members were localized at the plasma membrane.Chromosome localization analysis suggested that all members were distributed on 10 chromosomes,with 1-3 members on each chromosome.Besides,the promoter regions of the genes contained response elements for hormonal and adversity stresses.qRTPCR showed that MdTIP members were up-regulated in roots except MdTIP1;1,of which MdTIP1;3 and MdTIP1;4 were up-regulated 5.27 times and 5.69 times,respectively,compared with the control,suggesting that these two genes might be critical in response to drought stress.[Conclusion]Identification of MdTIP subfamily members is provided in this study.10 MdTIP members are differentially expressed in roots,stems,and leaves,and 12 members are highly expressed in roots.

关 键 词：苹果 水通道蛋白 TIPs亚家族基因 水分运输 生物信息学 

分 类 号：Q786[生物学—分子生物学] S661.1[农业科学—果树学]