草莓FaWRKY70基因克隆与表达模式分析  

Cloning and expression analysis of the FaWRKY70 gene in strawberry

作　　者：邵妍丽[1] 卢贝 贾思振[2] 汤伟华 廖云飞[1] SHAO Yanli;LU Bei;JIA Sizhen;TANG Weihua;LIAO Yunfei(Faculty of Agricultural Science and Technology,Xizang Vocational Technical College,Lhasa 850000,China;Faculty of Agronomy and Horticulture,Jiangsu Vocational College of Agriculture and Forestry,Jurong,Jiangsu 212400,China)

机构地区：[1]西藏职业技术学院农业科学技术学院,拉萨850000 [2]江苏农林职业技术学院农学园艺学院,江苏句容212400

出　　处：《西北植物学报》2024年第7期1105-1112,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：西藏自治区自然科学基金项目(XZ202201ZR0004G);江苏农林职业技术学院科技项目(2023kj23)。

摘　　要：【目的】WRKY是植物特异性转录因子家族之一,参与植物多种生命活动,但在草莓中鲜见WRKY70相关报道。深入解析WRKY70同源基因在草莓响应胁迫过程中的作用,加快分子育种技术应用,培育新草莓种质资源。【方法】用同源克隆法从‘红颜’草莓果实中克隆得到FaWRKY70基因,用生物信息学分析其保守结构域、理化性质、蛋白质结构及进化关系等,并结合qRT-PCR数据进行表达模式分析。【结果】FaWRKY70基因全长1020 bp,编码339个氨基酸;同源基因比对发现,FaWRKY70与苹果、牡丹等同科物种氨基酸序列相似度较高,且同源基因多与植物对生物、非生物胁迫响应相关,暗示FaWRKY70可能参与草莓抵御胁迫的过程;FaWRKY70在草莓不同器官中均有表达,且差异显著,在花中表达量最高,在果实中最低;水杨酸处理后,FaWRKY70基因快速响应,表达量在3 h后达到最高,随后逐渐降低;茉莉酸甲酯处理后FaWRKY70基因受诱导响应程度小,整体呈下调趋势。【结论】FaWRKY70能通过不同响应方式参与草莓生命活动及激素信号转导过程。[Objective]WRKY is one of the plant specific transcription factor families,involved in various plant life activities.However,currently there are few reports related to the WRKY70 gene in strawberry.Analyzing the role of WRKY70 homologous genes in strawberry in response to stress will help accelerate the application of molecular breeding technology and cultivate new strawberry germplasm resources.[Methods]The FaWRKY70 gene was cloned from‘Benihoppe’strawberry fruits using homologous cloning method.Its conserved domain,physicochemical properties,protein structure,and evolutionary relationship were analyzed using bioinformatics.Expression pattern analysis was performed using qRT-PCR.[Results]The FaWRKY70 gene had a length of 1020 bp and encodes 339 amino acids.Homologous gene alignment revealed that FaWRKY70 had a high degree of amino acid sequence similarity with species in the same family such as apple and peony.The homologous genes were mostly related to plant responses to biotic and abiotic stress,suggesting that FaWRKY70 may be involved in resistance to stress.FaWRKY70 was expressed in different organs of strawberry,with significant differences.The expression level was highest in flowers and lowest in fruits.Under salicylic acid treatment,the FaWRKY70 gene was quickly responded,reaching its highest expression level after 3 hours,and gradually decreased thereafter.Under the treatment with methyl jasmonate,the FaWRKY70 gene showed a slight degree of induction and an overall downward trend.[Conclusion]FaWRKY70 is involved in growth and hormone signal transduction through different response modes in strawberry.

关 键 词：草莓 FaWRKY70 基因克隆 差异表达 

分 类 号：Q785[生物学—分子生物学] Q786[农业科学—果树学] S668.4[农业科学—园艺学]