黑果腺肋花楸扦插生根特性及不定根发生模式研究  

Study on rooting characteristics and occurrence model of adventitious roots in cuttage of Aronia melanocarpa

作　　者：董立格 曲良谱[1] 张成霞[1] 韦庆翠[1] 李成忠[1] DONG Lige;QU Liangpu;ZHANG Chengxia;WEI Qingcui;LI Chengzhong(Jiangsu Agri-animal Husbandry Vocational College,Taizhou,Jiangsu 225300,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300

出　　处：《西北植物学报》2024年第7期1141-1152,共12页Acta Botanica Boreali-Occidentalia Sinica

基　　金：泰州市科技支撑计划(农业)项目(TN202007);江苏省林业科技示范与推广项目“江苏省林源药用植物资源发掘与创新利用关键技术长期科研基地”(LYKJ[2020]29);江苏农牧科技职业学院校级科研项目“黑果腺肋花楸生物学特性及其繁殖技术研究”(NSF201812)。

摘　　要：【目的】为了解黑果腺肋花楸扦插不定根起源及发育过程,揭示其扦插生根机理。【方法】以‘福康源1号’当年生半木质化插穗为研究材料,利用水培扦插技术和石蜡切片法对不定根形成过程中插穗内部组织结构及外部形态的变化规律进行观察。【结果】在水培条件下,IBA处理扦插生根期为30~40 d,扦插过程中皮孔处在10~15 d出现不定根,插穗切口处在15~20 d出现不定根,生根速度、不定根数量及根长均优于对照,外源诱导可显著提高生根率和生根质量。扦插前的插穗内无潜伏根原基存在,不定根原基在插后形成;不定根形成为愈伤组织生根型和内部分生组织生根型。皮部产生的不定根起源于维管形成层、韧皮薄壁细胞或皮层;愈伤组织产生的不定根是由愈伤组织内的薄壁细胞团特化而成;叶隙或枝隙是形成不定根原基和产生愈伤组织的主要区域。【结论】扦插生根属于多位点发生模式,属于诱导生根型。[Objective]The aim of the study is to investigate the origin and development of the adventitious roots in cuttage of Aronia melanocarpa as well as to understand its mechanism.[Methods]Taking the semi-lignified cuttings of‘Fukangyuan No.1’as materials,the changes in internal structure and external morphology of the cuttings during the formation of adventitious roots were observed by hydroponic cutting technology and paraffin section method.[Results]The results showed that the rooting period of IBAtreated cuttings was 30-40 d under hydroponic conditions.During the cutting process,the adventitious roots were appeared at the lenticel at 10-15 d and at at the cutting incision 15-20 d,and the rooting speed,number,and root length were better than the control.The number of adventitious roots in the cuttings was the largest and the rooting was fast.Exogenous induction significantly improved rooting rate and quality.There was no latent root primordium in the cuttings before cutting,which was formed after cutting. The adventitious roots were formed into callus rooting type and internal meristem rooting type. Theadventitious roots produced in the bark were originated from vascular cambium, phloem parenchyma cells,or cortex, while the adventitious roots produced by the callus were specialized by the parenchymal cells.Leaf gaps or branch gaps were the main areas for the formation of adventitious root primordia and the formationof callus. [Conclusion] The rooting of cuttings belongs to the multi-site mode and the induced rootingtype.

关 键 词：黑果腺肋花楸 扦插 不定根发生 叶隙 枝隙 

分 类 号：Q944.54[生物学—植物学] S792.25[农业科学—林木遗传育种]