利用冰冻切片机快速制作木质茎的显微切片技术  

A rapid cryo-sectioning method for micro-structural observation of woody stems

作　　者：马芳 宋玉霞[3] 刘文哲[1] MA Fang;SONG Yuxia;LIU Wenzhe(School of Life Science,Northwest University,Xian 710069,China;Institute of Ethnic Preparatory,Ningxia University,Yinchuan 750021,China;Center for Agricultural Biotechnology,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China)

机构地区：[1]西北大学生命科学学院,西安710069 [2]宁夏大学民族预科教育学院,银川750021 [3]宁夏农林科学院农业生物技术中心,银川750002

出　　处：《西北植物学报》2024年第7期1164-1168,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金面上项目(32371693);宁夏农林科学院农业高质量发展和生态保护科技创新示范项目“特色资源植物利用与沙产业技术研发”。

摘　　要：【目的】植物的木质茎因次生维管组织含量多、细胞壁木质化程度高、质地较硬,所以徒手切片和石蜡切片均难以获得满意的切片效果。冰冻切片机可对一定硬度的组织进行切片,是植物木质茎制片的有效工具之一。【方法】利用冰冻切片机对一些质地均匀、含水量较高的幼小木质茎直接切片;对木质坚硬、含水量较少的茎,经过FAA固定液固定、OCT包埋后(-30)~(-25)℃冰冻切片,再用0.1%甲苯胺蓝染色,显微镜观察并拍照染色效果。【结果】来源于乔木或灌木,直径不超过6 cm的木质茎,利用冰冻切片均可快速获得结构完整、图像清晰、色彩丰富的显微结构图像。【结论】冰冻切片机可用于幼小木质茎的显微切片制作,结合甲苯胺蓝染色,具有简便、高效、易操作的优点。[Objective]The woody stems of plants are difficult to obtain satisfactory microscopic image in both freehand and paraffin sections due to the high content of secondary vascular tissues and high degrees of lignification of cell walls.Microtome cryostat can section the tissue with a certain hardness.It will be one of the effective tools for the section of plant woody stems.[Methods]Some woody stems with uniform texture and high water content can be sectioned by cryo-sectioning without chemical fixation.For stems with hard wood and less water content,observation was performed after fixation with FAA and other fixation,OCT embedding,(-30)-(-25)℃sectioning,and staining with 0.1%toluidine blue staining solution.[Results]For tree or shrub stems with a diameter of less than 6 cm,cryo-sectioning method could be used to quickly obtain complete structure and clear and colorful microstructure images.[Conclusion]The microtome cryostat can be used for microstructure sectioning of woody stems combined with toluidine blue staining, which has the advantage of simplicity, efficiency, and ease of operation.

关 键 词：木质茎 冰冻切片 显微结构 

分 类 号：Q944-3[生物学—植物学]