健康与致病菌感染双峰驼肠道细菌和真菌群落的比较分析  

Diversity Analysis of Intestinal Bacterial and Fungal Communities between Healthy and Infected Bactrian Camels

作　　者：白茹 袁青青 张晓乐 吾尔恩·阿合别尔迪 木古丽·木哈西 BAI Ru;YUAN Qing-qing;ZHANG Xiao-le;WUEREN·Ahebieerdi;MUGULI·Muhaxi(College of Biological Sciences and Technology,Yili Normal University,Yining,Xinjiang,835000,China;Key Laboratory of Microbial Resources Protection Development and Utilization,Yili Normal University,Yining,Xinjiang,835000,China)

机构地区：[1]伊犁师范大学生物科学与技术学院,新疆伊宁835000 [2]伊犁师范大学微生物资源保护与开发利用重点实验室,新疆伊宁835000

出　　处：《新疆师范大学学报（自然科学版）》2024年第4期36-43,共8页Journal of Xinjiang Normal University(Natural Sciences Edition)

基　　金：伊犁师范大学微生物资源保护与开发利用重点实验室项目(YLUKLM2018003)。

摘　　要：文章利用高通量测序技术,分析4只新疆巴里坤县成年双峰驼粪便样本的细菌及真菌菌群多样性和结构组成,反映致病菌感染双峰驼肠道菌群特点及肠道菌群失衡与双峰驼健康的关系,并在细菌和真菌水平进行基因功能预测。结果表明,CG.101、CG.103和CG.104三个样本的微生物群落结构特征没有显著差异(P>0.05),例如细菌群落门、科、属水平上最大的分类类群均为:厚壁菌门、瘤胃球菌科、拟杆菌属;真菌优势门均为子囊菌门和担子菌门。与以上三个样本相比,CG.102样本微生物多样性和有益菌相对丰度均较低,条件致病菌、致病菌相对丰度较高。在细菌门水平上CG.102样本中厚壁菌门和拟杆菌门相对丰度较低,而变形菌门和放线菌门相对丰度较高;在细菌科水平上,阿克曼菌科、瘤胃球菌科、克里斯腾森菌科、理研菌科等相对丰度非常低;在细菌属水平上,阿克曼菌属、拟杆菌属、瘤胃球菌属、芽孢杆菌属相对丰度低于其他三个样本;在种水平上,该样本中检测出占比很高的绿脓杆菌和洛菲不动杆菌。CG.102样本真菌群落以单一的子囊菌门,囊菌科,丝胞菌属,多育赛多孢子菌为主。基因功能的预测说明CG.102骆驼免疫力下降并感染疾病。High-throughput sequencing technique was used to analyze the diversity and structural composition of bacterial and fungal flora in fecal samples of four adult bactrian camels in Barkun County,Xinjiang,reflect the characteristics of intestinal flora infected by pathogenic bacteria and the relationship between intestinal flora imbalance and bactrian camel health and the gene function was predicted at the bacterial and fungi level.The results showed that there was no significant differences in the microbial community structure characteristics among CG.101,CG.103 and CG.104 samples(P>0.05),for example,the largest taxonomic groups at the level of phylum,family and genus of bacterial community were Firmicutes,Ruminococcaceae and Bacteroides;The dominant phylum of fungi included Ascomycetes and Basidiomycetes.Compared with the above three samples,sample CG.102 had lower microbial diversity and relative abundance of beneficial bacteria,but more Conditional pathogens and pathogenic bacteria.At the Phylum level,sample CG.102 had lower abundance of Firmicutes and Bacteroides,and higher abundance of Proteobacteria and Actinobacteria.At the family level,the relative abundant of Akkermansia,Ruminococcaceae,Christensenellaceae,Rikenellaceae was significantly lower;At the genus level,the relative abundance of Akkermansia,Bacteroides,Ruminococcaceae and Bacillus was obviously lower than that of the other three samples;At the species level,a high proportion of Pseudomonas aeruginosa and Acithrobacter lwoffii were detected in sample CG.102.The fungal community of sample CG.102 was dominated by a single Ascomycota,Ascomycodaceae,Hyphomycetes,Scedosporium prolificans.The prediction of bacterial indicated that the camel represented by CG.102 sample have decreased immunity and be infected with diseases.

关 键 词：高通量测序 粪便 菌群失衡 细菌多样性 真菌多样性 

分 类 号：Q938.1[生物学—微生物学]