GC-MS法测定复方鲜竹沥液中特征性成分α-金合欢烯含量及制剂中生姜提取工艺研究  

The GC-MS Method Determination the Content of the Characteristic Componentα-farnesene in the Fufang Xianzhuli Ye and Study the Ginger Extraction Process

作　　者：黄东 吴毅 丁银平 朱徐东 朱娜 肖小武 付辉政 HUANG Dong;WU Yi;DING Yinping;ZHU Xudong;ZHU Na;XIAO Xiaowu;FU Huizheng(Jiangxi Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Nanchang Jiangxi 330029,China;Jiangxi University of Chinese medicine,Nanchang Jiangxi 330004,China)

机构地区：[1]江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品与医疗器械质量工程技术研究中心,江西南昌330029 [2]江西中医药大学,江西南昌330004

出　　处：《药品评价》2024年第3期281-285,共5页Drug Evaluation

基　　金：江西省重大科技研发专项(20223AAG02020)。

摘　　要：目的 建立生姜特征性成分α-金合欢烯的GC-MS含量测定方法并研究生姜的提取工艺。方法 利用气质联用技术(GC-MS)对复方鲜竹沥液和生姜挥发油溶液的挥发性成分进行对比分析,找到生姜特征成分α-金合欢烯;将α-金合欢烯作为指标,建立其含量测定方法;研究处方中生姜不同生产工艺α-金合欢烯的含量及转移率。结果 复方鲜竹沥液和生姜挥发油溶液分别鉴定出24个和20个挥发性成分,并得到生姜特征成分α-萜品醇和α-金合欢烯。α-金合欢烯的GC-MS方法学专属性强、线性、精密度、稳定性、重复性均良好,回收率符合要求。测定不同生产工艺的生姜汁中α-金合欢烯含量,发现榨汁提取工艺对生姜中挥发性成分的保留至关重要。结论 该方法稳定可靠,可为优化复方鲜竹沥液中生姜制备工艺提供实验依据。Objective To establish a GC-MS quantitative method for the characteristic constituentα-farnesene of ginger,and study the ginger extraction process in fufang xianzhuli ye.Methods In this experiment,the volatile components of fufang xianzhuli ye and ginger volatile oil solution were compared and analyzed by GC-MS,and the characteristic componentsα-terpineol andα-farnesene of ginger were found.Established a GC-MS method to determine the contents ofα-farnesene in fufang xianzhuli ye and studed the contents and transfer rates ofα-farnesen in ginger with different production processes.Results The fufang xianzhuli ye and ginger volatile oil solution identified 24 and 20 volatile components,respectively.The results of the GC-MS method showed that the exclusivity,linearity,precision,stability and repeatability of the method were good,and the recovery rate met the requirements.By measuring the contents ofα-farnesene in ginger with different production processes,it was found that the extraction process of the juice was very important for the retention of volatile components in ginger.Conclusion This method is stable and reliable,and can provide experimental basis for optimizing the preparation process of ginger in fufang xianzhuli ye.

关 键 词：GC-MS 复方鲜竹沥液 α-金合欢烯 生姜 

分 类 号：R286.0[医药卫生—中药学]