TGFβ1/Smads信号通路抗纤维化抑制心室重构及红花黄色素干预作用的研究  

Hydroxysafflor Yellow Alleviates Ventricular Remodeling in Myocardial Infarction by Regulating the TGFβ1/Smads Signaling Pathway

作　　者：周芳丽[1] 陈鲲翰 刘春花[2] 王晓敏[2] 肖锐[1] ZHOU Fangi;CHEN Kunhan;LIU Chunhua;WANG Xiaomin;XIAO Rui(Nanchang Hongdu Hospital of TCM,Nanchang Jiangxi 330006,China;Jiangxi University of Chinese Medicine,Nanchang Jiangxi 330004,China)

机构地区：[1]南昌市洪都中医院,江西南昌330006 [2]江西中医药大学,江西南昌330004

出　　处：《药品评价》2024年第3期290-293,共4页Drug Evaluation

基　　金：江西省卫生健康委科技项目(202211677);江西省教育厅科学技术研究项目(GJJ2200940);江西中医药大学大学生创新项目(X202310412194)。

摘　　要：目的 研究红花黄色素对心肌梗死后的心室重构及TGFβ1/Smads信号通路的影响。方法 结扎左冠状动脉前降支建立心肌梗死模型,假手术组开胸显露左冠状动脉前降支但不行结扎。建模成功大鼠随机分为模型组,红花黄色素高、中、低剂量组,氯沙坦组,给药4周。观察心脏形态结构变化;分析血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧物酶(GSH-Px)和过氧化氢酶(CAT)含量变化;Westem blot检测心肌组织转化生长因子β1(TGFβ1)、SMAD2/3、P-SMAD2/3蛋白表达量。结果 与假手术组比较,模型组心脏体积变大,梗死区面积大,左室壁凹陷,变薄;与模型组比较,给药组左心室壁没有明显凹陷,梗死区范围和体积变小;血清MDA降低,SOD、GSH-Px和CAT活性增强(P<0.05);与模型组比较,给药组心肌组织TGFβ1及SMAD2/3蛋白表达下降(P<0.05)。结论 红花黄色素能够减轻心室重构,缓解氧化应激,可能是通过抑制TGFβ1/Smads信号通路而实现。Objective To investigate the effects of hydroxysafflor yellow on ventricular remodeling and the TGFβ1/Smads signaling pathway after myocardial infarction.Methods Myocardial infarction model was established by ligating the left anterior descending coronary artery,with sham surgery group as control.Rats were randomly divided into model group,low,medium and high dose hydroxysafflor yellow groups,and losartan group.Rats were dosed for 4 weeks.Changes of cardiac morphology were observed.The content of malonaldehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT)in serum of rats in each group were analyzed.The expression of transforming growth factor(TGFβ1),SMAD2/3,and P-SMAD2/3 in myocardial tissue were detected by western blot.Results Compared with the sham-operated group,the model group increased cardiac volume,larger infarct area,concave and thinning left ventricular wall.Compared with the model group,the treatment groups exhibited no obvious concavity in the left ventricular wall,reduced infarct area and volume;decreased serum MDA levels,increased activities of SOD,GSH-Px,and CAT(P<0.05).Compared with the model group,the treatment groups decreased protein expression of TGFβ1 and P-SMAD2/3 in cardiac tissue(P<0.05).Conclusion Hydroxysafflor yellow could alleviate ventricular remodeling,relieve oxidative stress,inhibit the TGFβ1/Smads signaling pathway.

关 键 词：红花黄色素 心室重构 TGFβ1/Smads信号通路 抗氧化 

分 类 号：R285.5[医药卫生—中药学]