发酵虫草菌粉中三种核苷成分的含量测定  

Determination of Three Nucleoside Components in Fermentative Cordycepic Fungal Powder

作　　者：郑海华 孟周钧 邓愍民 ZHENG Haihua;MENG Zhoujun;DENG Minmin(Jiangxi Guoyao Pharmaceutical Ltd.Liablity Co.,Nanchang Jiangxi 330052,China)

机构地区：[1]江西国药有限责任公司,江西南昌330052

出　　处：《药品评价》2024年第3期294-297,共4页Drug Evaluation

摘　　要：目的对发酵虫草菌粉三种核苷成分(尿苷、鸟苷、腺苷)的含量及其聚类比例关系进行研究,旨在为其质量标准提升与新产品开发提供参考。方法采用Waters 2489高效液相色谱仪;色谱柱:Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm);流动相:A相为甲醇,B相为0.01mol/L磷酸二氢钾溶液;梯度洗脱;柱温:25℃;检测波长:260 nm;流速:1.0 mL/min;进样量:10μL。结果尿苷、鸟苷、腺苷分别在0.1275~50.9896μg/mL(r=0.9999)、0.1111~44.4595μg/mL(r=0.9999)、0.1351~54.0553μg/mL(r=0.9999)范围内线性关系良好;平均加样回收率(n=6)分别为97.89%(RSD=0.13%)、103.69%(RSD=3.48%)、95.92%(RSD=2.11%)。结论本法操作简便、稳定可靠、灵敏度高、专属性好,可用于发酵虫草菌粉中三种核苷成分含量的准确检测。Objective The content and cluster ratio of the three nucleosides(uridine,guanosine and adenosine)of fermentative cordycepic fungal powder were studied,aiming to provide reference for the quality standard improvement and the development of new products.Methods Using Waters 2489 high performance liquid chromatograph;column:Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm);mobile phase:phase A was methanol,and phase B was 0.01 mol/L potassium dihydrogen phosphate solution;gradient elution;column temperature:25℃;detection wavelength:260 nm;flow rate:1.0 mL/min;sample quantity:10μL.Results Uridine,guanosine,and adenosine had good linear relationships within the ranges of 0.1275-50.9896μg/mL(r=0.9999),0.1111-44.4595μg/mL(r=0.9999),and 0.1351-54.0553μg/mL(r=0.9999)respectively.The average recovery rates(n=6)were 97.89%(RSD=0.13%),103.69%(RSD=3.48%),and 95.92%(RSD=2.11%)respectively.Conclusion The method is simple to operate,stable and reliable,has high sensitivity and good specificity,and can be used to accurately detect the content of three nucleoside components in fermentative cordycepic fungal powder.

关 键 词：发酵虫草菌粉 高效液相色谱法 核苷 

分 类 号：R284.1[医药卫生—中药学]