^(99)Tc^(m)-DTPA-CGRRAGGSC在非小细胞肺癌裸鼠模型的显像研究  

Imaging study on the ^(99)Tc^(m)-DTPA-CGRRAGGSC scintigraphy in the nude mice model with non-small cell lung cancer

作　　者：李卫鹏 李辉 刘春艳 沈如月 訾刚 袁超 LI Weipeng;LI Hui;LIU Chunyan;SHEN Ruyue;ZI Gang;YUAN Chao(Department of Nuclear Medicine,The First Affiliated Hospital of Bengbu Medical University,Bengbu Anhui 233004;Catheter Room,Bengbu Hospital of Traditional Chinese Medicine,Bengbu Anhui 233080,China)

机构地区：[1]蚌埠医科大学第一附属医院核医学科,安徽蚌埠233004 [2]安徽省蚌埠市中医院导管室,233080

出　　处：《蚌埠医学院学报》2024年第6期701-705,共5页Journal of Bengbu Medical College

基　　金：蚌埠医学院自然科学研究重点项目(2020byzd097)。

摘　　要：目的:探讨放射性核素^(99)Tc^(m)标记环九肽(CGRRAGGSC)对荷非小细胞肺癌裸鼠的显像研究。方法:用^(99)Tc^(m)标记DTPA-CGRRAGGSC,测定其标记率及稳定性,制备荷非小细胞肺癌小鼠模型,经荷瘤鼠尾静脉注射标记物2 h后进行SPECT静态显像,并计算出靶与非靶(T/NT)比值。结果:成功制备出^(99)Tc^(m)-DTPA-CGRRAGGSC,在PBS和人血清中37℃放置6 h时放射性化学纯度在90%以上;经荷瘤鼠尾静脉注射标记物2 h后T/NT比值最高达3.5。结论:^(99)Tc^(m)标记DTPA-CGRRAGGSC制备简便,其对荷非小细胞肺癌裸鼠具有较高靶向性,^(99)Tc^(m)-DTPA-CGRRAGGSC有可能在IL-11Rα高表达的非小细胞肺癌分子显像中发挥重要作用。Objective:To explore the imaging of scintigraphy of CGRRAGGSC labeled with ^(99)Tc^(m) in nude mice with non-small cell lung cancer.Methods:The DTPA-CGRRAGGSC was labeled with ^(99)Tc^(m),and the labeling rate and stability were measured.The mice model with non-small cell lung cancer were established.The markers were injected into the tail vein of the tumor-bearing mice,the SPECT static imaging was performed after 2 hours,and the ratio of target to non-target(T/NT)was calculated.Results:The ^(99)Tc^(m)-DTPA-CGRRAGGSC was successfully radiolabeled,and the radiochemical purity of which was above 90%when it was placed in PBS and human serum at 37℃for 6 h.The T/NT ratio was as high as 3.5 after 2 hours of injection of the marker through the tail vein of tumor-bearing mice.Conclusions:The ^(99)Tc^(m)-labeled DTPA-CGRRAGGSC is easy to be prepared,and it has high targeting to nude mice with non-small cell cancer.^(99)Tc^(m)-DTPA-CGRRAGGSC may play an important role in molecular imaging of non-small cell lung cancer with high expression of IL-11Rα.

关 键 词：肺肿瘤 ^(99)Tc^(m)标记环九肽 白细胞介素-11Rα 放射性核素标记 

分 类 号：R817.4[医药卫生—影像医学与核医学]