朱砂叶螨V-ATP酶D亚基基因克隆与表达分析  

Cloning and expression analysis of V-ATPase-D subunit gene of Tetranychus cinnabarinus

作　　者：耿文 王谦稳 胡祺凡 陈佳雯 卜春亚[1] GENG Wen;WANG Qianwen;HU Qifan;CHEN Jiawen;BU Chunya(College of Biological and Resource Environment/Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]北京农学院生物与资源环境学院/农业农村部华北都市农业重点实验室,北京102206

出　　处：《北京农学院学报》2024年第3期1-5,共5页Journal of Beijing University of Agriculture

基　　金：国家自然科学基金资助项目(31670648);北京市自然科学基金资助项目(6212004)。

摘　　要：【目的】旨在寻找防治朱砂叶螨有效的生物学办法。【方法】克隆出朱砂叶螨V-ATP酶D亚基(V-ATPase-D)基因,并对该基因进行了生物学特征分析;采用荧光定量PCR技术,对朱砂叶螨其生命周期中V-ATPase-D基因表达量进行了测定。【结果】克隆出朱砂叶螨V-ATPase-D(GenBank登录号:OR836605)。朱砂叶螨V-ATPase-D基因全长956 bp,开放阅读框744 bp,编码247个氨基酸,V-ATPase-D在朱砂叶螨的不同生长发育阶段均表现出了基因活性,其中在成螨期内展现出最高的表达水平,其次是卵阶段,而若螨与幼螨阶段则呈现出较低的基因表达水平。【结论】成功克隆出朱砂叶螨V-ATPase-D基因,并明确其在朱砂叶螨生长过程中不同时期内的基因表达活性,为进一步探索基因的作用机制同时也对以V-ATP酶为靶标的新型生物杀螨剂提供了研究基础。[Objective]The study aimed to identify effective biological approaches for controlling Tetranychus cinnabarinus.[Methods]The V-ATPase-D gene was cloned and its gene sequence was analyzed;real-time quantitative fluorescence PCR was used to characterize the expression of V-ATPase-D.[Results]The full length of the cloned V-ATPase-D subunit gene(GenBank accession number:OR836605)is 956 bp,with an open reading frame of 744 bp encoding 247 amino acids.The expression of V-ATPase-D was highest in the adult mite stage followed by the egg stage,and lowest in the nymph and larva stages.[Conclusion]The V-ATPase-D gene was cloned and its expression characteristics at various stages were clarified,which may provide basis for the development of new biopesticidal acaricides targeting the V-ATPase in the future.

关 键 词：朱砂叶螨 V-ATP酶 克隆 荧光定量PCR 

分 类 号：S433.7[农业科学—农业昆虫与害虫防治]