侧流免疫层析定量检测方法的研究进展  

Research progress on quantitative detection methods of lateral flow immunochromatography assay

作　　者：顾佳钰 胡馨儿 王晓飞 张颖 张海 曹岩 GU Jiayu;HU Xiner;WANG Xiaofei;ZHANG Ying;ZHANG Hai;CAO Yan(Department of Pharmacy,Naval Medical University,Shanghai 200433,China;School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;The First Maternal and Infant Health Hospital Affiliated to Tongji University,Shanghai 200040,China)

机构地区：[1]海军军医大学药学系,上海200433 [2]上海理工大学健康科学与工程学院,上海200093 [3]同济大学附属第一妇婴保健院,上海200040

出　　处：《药学实践与服务》2024年第7期273-277,284,共6页Journal of Pharmaceutical Practice and Service

基　　金：国家自然科学基金资助项目(82174092);上海市科委资助项目(21ZR1483000);上海市浦江计划资助项目(21PJD083)。

摘　　要：侧流免疫层析技术是一项新型的即时检测技术,采用层析膜和标记材料进行检测。该检测技术具有方便快捷、成本低廉的特点,因此被广泛应用于生物医药、疾病检测、食品安全、环境保护等多个领域。传统的侧流免疫层析需要使用肉眼观测,因此只能提供至多半定量的结果,并且有可能存在观测误差。随着各种类型的标记材料和灵敏检测仪器的出现及广泛应用,侧流免疫层析技术逐步实现了对待检测物的定量检测。通过综述识别元件、标记材料、检测仪器等,阐述侧流免疫层析检测系统的研究进展以及目前的应用情况。Lateral flow immunochromatography assay is a new instantaneous detection technology that employs a chromatographic membrane and labeling materials for detection.This detection technology is convenient,fast,and inexpensive,and is therefore widely used in a number of different fields,such as biomedicine,disease detection,food safety,environmental protection,and so on.Traditional lateral flow immunochromatography assay relied on visual observation and provided only qualitative or semi-quantitative results.By utilizing various types of markers and sensitive detection devices,lateral flow immunochromatography assay enables quantitative and multi-component detection of the analytes.The research progress on the lateral flow immunoassay detection system and its current applications in the context of recognition elements,labeling materials,and detection instruments were reviewed in this paper.

关 键 词：侧流免疫层析 检测仪 生物标记 

分 类 号：Q503[生物学—生物化学]