Identification of P-type plasma membrane H^(+)-ATPases in common wheat and characterization of TaHA7 associated with seed dormancy and germination  

作　　者：Bingli Jiang Wei Gao Yating Jiang Shengnan Yan Jiajia Cao Litian Zhang Yue Zhang Jie Lu Chuanxi Ma Cheng Chang Haiping Zhang 

机构地区：[1]Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow&Huai River Valley,Ministry of Agriculture and Rural Affairs/College of Agronomy,Anhui Agricultural University,Hefei 230036,China

出　　处：《Journal of Integrative Agriculture》2024年第7期2164-2177,共14页农业科学学报（英文版）

基　　金：supported by grants from the University Synergy Innovation Program of Anhui Province,China(GXXT-2021-058);the National Natural Science Foundation of China(U20A2033);the Natural Science Foundation of Anhui Province,China(2108085MC98);the Key Scientific and Technological Breakthroughs of Anhui Province,China(2021d06050003);the Anhui Province Education Department Sciences Research Project,China(YJS20210212);the Scientific Research Project of Higher Education in Anhui Province,China(2022AH050924 and 2022AH050885);the Jiangsu Collaborative Innovation Center for Modern Crop Production,China(JCIC-MCP)。

摘　　要：The P-type plasma membrane(PM)H^(+)-ATPases(HAs)are crucial for plant development,growth,and defense.The HAs have been thoroughly characterized in many different plants.However,despite their importance,the functions of HAs in germination and seed dormancy(SD)have not been validated in wheat.Here,we identified 28 TaHA genes(TaHA1-28)in common wheat,which were divided into five subfamilies.An examination of gene expression in strong-and weak-SD wheat varieties led to the discovery of six candidate genes(TaHA7/-12/-14/-16/-18/-20).Based on a single nucleotide polymorphism(SNP)mutation(C/T)in the TaHA7 coding region,a CAPS marker(HA7)was developed and validated in 168 wheat varieties and 171 Chinese mini-core collections that exhibit diverse germination and SD phenotypes.We further verified the roles of the two allelic variations of TaHA7 in germination and SD using wheat mutants mutagenized with ethyl methane sulphonate(EMS)in‘Jimai 22’and‘Jing 411’backgrounds,and in transgenic Arabidopsis lines.TaHA7 appears to regulate germination and SD by mediating gibberellic acid(GA)and abscisic acid(ABA)signaling,metabolism,and biosynthesis.The results presented here will enable future research regarding the TaHAs in wheat.

关 键 词：wheat P-type plasma membrane H^(+)-ATPase seed dormancy abscisic acid GIBBERELLIN 

分 类 号：S512.1[农业科学—作物学]