Genetic analysis and candidate gene identification of salt tolerancerelated traits in maize  

作　　者：Hui Fang Xiuyi Fu Hanqiu Ge Mengxue Jia Jie Ji Yizhou Zhao Zijian Qu Ziqian Cui Aixia Zhang Yuandong Wang Ping Li Baohua Wang 

机构地区：[1]Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region,Ministry of Agriculture and Rural Affairs/School of Life Sciences,Nantong University,Nantong 226019,China [2]Maize Research Institute,Beijing Academy of Agriculture and Forestry Sciences(BAAFS),Beijing 100097,China

出　　处：《Journal of Integrative Agriculture》2024年第7期2196-2210,共15页农业科学学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(32101730);the National Key R&D Program Projects,China(2021YFD1201005);the Beijing Academy of Agriculture and Forestry Sciences(BAAFS);Excellent Scientist Training Program,China(JKZX202202);the BAAFS Science and Technology Innovation Capability Improvement Project,China(KJCX20230433)。

摘　　要：Soil salinization poses a threat to maize production worldwide,but the genetic mechanism of salt tolerance in maize is not well understood.Therefore,identifying the genetic components underlying salt tolerance in maize is of great importance.In the current study,a teosinte-maize BC2F7 population was used to investigate the genetic basis of 21 salt tolerance-related traits.In total,125 QTLs were detected using a high-density genetic bin map,with one to five QTLs explaining 6.05–32.02%of the phenotypic variation for each trait.The total phenotypic variation explained(PVE)by all detected QTLs ranged from 6.84 to 63.88%for each trait.Of all 125 QTLs,only three were major QTLs distributed in two genomic regions on chromosome 6,which were involved in three salt tolerance-related traits.In addition,10 pairs of epistatic QTLs with additive effects were detected for eight traits,explaining 0.9 to 4.44%of the phenotypic variation.Furthermore,18 QTL hotspots affecting 3–7 traits were identified.In one hotspot(L5),a gene cluster consisting of four genes(ZmNSA1,SAG6,ZmCLCg,and ZmHKT1;2)was found,suggesting the involvement of multiple pleiotropic genes.Finally,two important candidate genes,Zm00001d002090 and Zm00001d002391,were found to be associated with salt tolerance-related traits by a combination of linkage and marker-trait association analyses.Zm00001d002090 encodes a calcium-dependent lipid-binding(CaLB domain)family protein,which may function as a Ca^(2+)sensor for transmitting the salt stress signal downstream,while Zm00001d002391 encodes a ubiquitin-specific protease belonging to the C19-related subfamily.Our findings provide valuable insights into the genetic basis of salt tolerance-related traits in maize and a theoretical foundation for breeders to develop enhanced salt-tolerant maize varieties.

关 键 词：MAIZE salt tolerance-related traits QTL mapping region-based association analysis 

分 类 号：S513[农业科学—作物学]