The biosynthesis of alarm pheromone in the wheat aphid Rhopalosiphum padi is regulated by hormones via fatty acid metabolism  

作　　者：Chengxian Sun Yaoguo Qin Julian Chen Zhengxi Li 

机构地区：[1]Department of Entomology/Ministry of Agriculture Key Laboratory for Monitoring and Environment-friendly Control of Crop Pests,College of Plant Protection,China Agricultural University,Beijing 100193,China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China

出　　处：《Journal of Integrative Agriculture》2024年第7期2346-2361,共16页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31972267 and 3227253);the Chinese Universities Scientific Fund(2023TC109)。

摘　　要：Aphids are major insect pests in agriculture and forestry worldwide.Following attacks by natural enemies,many aphids release an alarm pheromone to protect their population.In most aphids,the main component of the aphid alarm pheromone(AAP)is the sesquiterpene hydrocarbon(E)-β-farnesene(EβF).However,the mechanisms behind its biosynthesis and regulation remain poorly understood.In this study,we used the bird cherry–oat aphid Rhopalosiphum padi,which is an important wheat aphid,to investigate the regulatory mechanisms of EβF biosynthesis.Our results showed that EβF biosynthesis occurs during the mature embryo period and the molting period of the 1st-and 2nd-instar nymphs.Triglycerides provide the prerequisite material for EβF production and release.Based on transcriptome sequencing,RNAi analysis,hormone treatments,and quantitative measurements,we found that the biosynthesis of EβF utilizes acetyl coenzyme A produced from fatty acid degradation,which can be suppressed by juvenile hormone but it is promoted by 20-hydroxyecdysone through the modulation of fatty acid metabolism.This is the first systemic study on the modulation of EβF production in aphids.The results of our study provide insights into the molecular regulatory mechanisms of AAP biosynthesis,as well as valuable information for designing potential aphid control strategies.

关 键 词：(E)-β-farnesene critical period for biosynthesis fatty acid metabolism juvenile hormone 20-HYDROXYECDYSONE 

分 类 号：S435.122.2[农业科学—农业昆虫与害虫防治]