Genetic and pathogenic characterization of new infectious bronchitis virus strains in the GVI-1 and GI-19 lineages isolated in central China  

作　　者：Yuhan Yang Dou Wang Yaning Bai Wenyan Huang Shimin Gao Xingchen Wu Ying Wang Jianle Ren Jinxin He Lin Jin Mingming Hu Zhiwei Wang Zhongbing Wang Haili Ma Junping Li Libin Liang 

机构地区：[1]College of Veterinary Medicine,Shanxi Agricultural University,Jinzhong 030801,China [2]Shanxi Provincial Animal Disease Prevention and Control Center,Taiyuan 030000,China

出　　处：《Journal of Integrative Agriculture》2024年第7期2407-2420,共14页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32202788);the Special Research Fund of Shanxi Agricultural University for High-level Talents,China(2021XG004);the Fund for Shanxi“1331 Project”,China(20211331-13);the Shanxi Province Excellent Doctoral Work Award-Scientific Research Project,China(SXBYKY2021063,SXBYKY2021005,and SXBYKY 2022014);the earmarked fund for Modern Agro-industry Technology Research System of Shanxi Province,China(2023CYJSTX15-13);the Fundamental Research Program of Shanxi Province,China(202103021224156)。

摘　　要：Avian infectious bronchitis(IB)is a highly contagious infectious disease caused by infectious bronchitis virus(IBV),which is prevalent in many countries worldwide and causes serious harm to the poultry industry.At present,many commercial IBV vaccines have been used for the prevention and control of IB;however,IB outbreaks occur frequently.In this study,two new strains of IBV,SX/2106 and SX/2204,were isolated from two flocks which were immunized with IBV H120 vaccine in central China.Phylogenetic and recombination analysis indicated that SX/2106,which was clustered into the GI-19 lineage,may be derived from recombination events of the GI-19 and GI-7 strains and the LDT3-A vaccine.Genetic analysis showed that SX/2204 belongs to the GVI-1 lineage,which may have originated from the recombination of the GI-13 and GVI-1 strains and the H120 vaccine.The virus cross-neutralization test showed that the antigenicity of SX/2106 and SX/2204 was different from H120.Animal experiments found that both SX/2106 and SX/2204 could replicate effectively in the lungs and kidneys of chickens and cause disease and death,and H120 immunization could not provide effective protection against the two IBV isolates.It is noteworthy that the pathogenicity of SX/2204 has significantly increased compared to the GVI-1 strains isolated previously,with a mortality rate up to 60%.Considering the continuous mutation and recombination of the IBV genome to produce new variant strains,it is important to continuously monitor epidemic strains and develop new vaccines for the prevention and control of IBV epidemics.

关 键 词：infectious bronchitis virus GI-19 lineage GVI-1 lineage complete genome recombination PATHOGENICITY 

分 类 号：S852.65[农业科学—基础兽医学]