基于miR-29b/TGF-β/Smad信号通路探讨复方血栓通对糖尿病视网膜病变大鼠作用的机制 分析  

Mechanism of Fufang Xueshuantong on diabetic retinopathy in rats based on miR-29b/TGF-β/Smad signaling pathway

作　　者：蒲洁琨 姜爱雯[1] 冯筱巍[1] 庞茜茜 褚明娟 汤建华[1] PU Jie-kun;JIANG Ai-wen;FENG Xiao-wei(The First Affiliated Hospital of Hebei North University,Zhangjiakou Hebei 075000,China)

机构地区：[1]河北北方学院附属第一医院药学部,河北张家口075000

出　　处：《临床和实验医学杂志》2024年第11期1121-1125,共5页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金(编号:8180150874);张家口市重点研发计划项目(编号:2221168D)。

摘　　要：目的基于miR-29b/转化生长因子β(TGF-β)/Smad信号通路探讨复方血栓通对糖尿病视网病变(DR)大鼠的的作用机制。方法100只雄性SD大鼠,其中80只建立糖尿病模型,20只作为正常组。通过腹腔注射1%链脲佐菌素(STZ)成功建立糖尿病DR大鼠模型72只,按照随机数字表法将其分为模型组、二甲双胍组、复方血栓通1组、复方血栓通2组,每组各18只。二甲双胍组通过灌胃二甲双胍184 mg/kg,复方血栓通1组通过灌胃复方血栓通片100 mg/kg,复方血栓通2组通过灌胃复方血栓通片200 mg/kg,给药10周。正常组大鼠随意给予正常饮食和饮用水,而模型组、二甲双胍组、复方血栓通1组、复方血栓通2组大鼠喂以高脂肪饮食,直至第16周末。测定并比较各组大鼠血清胰岛素与血糖水平,比较各组大鼠血清细胞因子[肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β和IL-6]、超氧化物歧化酶(SOD)、丙二醛水平。实时荧光定量PCR与蛋白印迹法分别检测各组大鼠视网膜组织中miR-29b表达与TGF-β/Smad信号通路蛋白表达。结果与正常组相比,模型组大鼠血清胰岛素及30、60、90 min内血糖均明显升高,差异均有统计学意义(P<0.05);与模型组比较,二甲双胍组、复方血栓通1组、复方血栓通2组大鼠血清胰岛素及30、60、90 min内血糖水平均显著降低,差异均有统计学意义(P<0.05)。与正常组相比,模型组大鼠血清TNF-α、IL-1β和IL-6水平均显著升高,差异均有统计学意义(P<0.05);与模型组比较,二甲双胍组、复方血栓通1组、复方血栓通2组大鼠血清TNF-α、IL-1β和IL-6水平均显著降低,差异均有统计学意义(P<0.05)。与正常组相比,模型组血清中的血清SOD活性显著降低,丙二醛含量显著升高,差异均有统计学意义(P<0.05);与模型组比较,二甲双胍组、复方血栓通1组、复方血栓通2组大鼠血清SOD活性显著增加,丙二醛含量降低,差异均有统计学意义(P<0.05)。�Objective To explore the mechanism of Fufang Xueshuantong on diabetes retinopathy(DR)in rats based on miR-29b/transforming growth factor-β(TGF-β)/Smad signaling pathway.Methods Among 100 male SD rats,80 rats were used to establish diabetes model,and 20 rats were used as normal group.72 diabetes DR rats were successfully established by intraperitoneal injection of 1%streptozotocin(STZ).They were divided into the model group,the metformin group,the compound Xueshuantong 1 group and the compound Xueshuantong 2 groups according to the random number table method,with 18 rats in each group.The metformin group was treated with 184 mg/kg of metformin by gavage,the Fufang Xueshuantong 1 group was treated with 100 mg/kg of Fufang Xueshuantong Tablets,and the Fufang Xueshuantong 2 group was treated with 200 mg/kg of Fufang Xueshuantong Tablets for 10 weeks.The normal group rats were given normal diet and drinking water at will,while the model group,metformin group,compound Xueshuantong 1 group,and compound Xueshuantong 2 group rats were fed a high-fat diet until the 16th weekend.The levels of serum insulin and blood glucose in each group of rats were measured and compared,and the levels of serum cytokines[tumor necrosis factor-α(TNF)-α,interleukin(IL)-1βand IL-6],superoxide dismutase(SOD),and malondialdehyde in each group of rats were compared.The expression of miR-29b and TGF-β/Smad signaling pathway protein expression in retinal tissues of rats in each group were detect using real time fluorescence quantitative PCR and Western blotting,respectively.Results Compared with the normal group,the serum insulin and blood glucose levels within 30,60,and 90 minutes in the model group rats were significantly increased,and the differences were statistically significant(P<0.05);compared with the model group,the serum insulin and blood glucose levels within 30,60,and 90 minutes of rats in the metformin group,compound Xueshuantong 1 group,and compound Xueshuantong 2 group were significantly reduced,and the differences were statis

关 键 词：大鼠 复方血栓通 糖尿病视网膜病变 miR-29b 转化生长因子Β 

分 类 号：R285.5[医药卫生—中药学]