miR-3191-5p在早期激素性股骨头坏死患者中的诊断价值分析  

Analysis on the diagnostic value of miR-3191-5p in patients with early steroid-induced avascular necrosis of femoral head

作　　者：张路 胡丽萍 ZHANG Lu;HU Liping(Department of Orthopedic Surgery,Jianli People’s Hospital,Hubei,Jianli 433300,China;Department of Clinical Laboratory,Jianli People’s Hospital,Hubei,Jianli 433300,China)

机构地区：[1]湖北省监利市人民医院骨外科,湖北监利433300 [2]湖北省监利市人民医院检验科,湖北监利433300

出　　处：《中国医药科学》2024年第12期175-178,共4页China Medicine And Pharmacy

摘　　要：目的检测并探讨外周血中微小RNA(miRNA)-3191-5p在激素性股骨头坏死患者中的表达以及诊断价值。方法前瞻性选取2017年3月至2023年2月于监利市人民医院就诊的201例疑似激素性股骨头坏死患者,分为确诊组(99例)和未确诊组(102例);同期选取体检就诊的82例健康人群作为对照组。分别检测并比较三组外周血中miR-3191-5p相对含量,骨密度(BMD)和骨钙素(BGP)水平,分析miR-3191-5p与BMD、BGP的相关性,采用受试者工作曲线(ROC)评估miR-3191-5p在激素性股骨头的诊断价值。结果确诊组血清miR-3191-5p相对表达水平低于未确诊组和对照组,BMD和BGP高于未确诊组和对照组。确诊组轻中度组miR-3191-5p相对表达水平高于重度组,BMD和BGP低于重度组;且miR-3191-5p分别与BMD、BGP均呈显著负相关(P<0.05)。miR-3191-5p区分轻中度与非轻中度、重度和非重度、激素性股骨头坏死和非该病患者的ROC下面积和分别为0.764、0.916和0.911。结论血清miR-3191-5p能够作为激素性股骨头坏死患者早期诊断的敏感生物标志物。Objective To detect and investigate the expression of microRNA(miRNA)-3191-5p in peripheral blood of patients with steroid-induced avascular necrosis of femoral head(SANFH)and its diagnostic value.Methods A total of 201 patients with suspected SANFH admitted to Jianli People’s Hospital from March 2017 to February 2023 were prospectively selected and divided into the confirmed group(n=99)and the undiagnosed group(n=102).In the same period,healthy individuals who underwent physical examinations were selected as the control group(n=82).The relative content of miR-3191-5p,bone mineral density(BMD)and bone gla-protein(BGP)in peripheral blood of three groups were detected and compared,respectively.The correlations between miR-3191-5p and BMD and BGP were analyzed.The receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of miR-3191-5p in steroid-induced femoral head.Results The relative expression level of serum miR-3191-5p in the confirmed group was lower than that in the undiagnosed group and the control group,while BMD and BGP were higher than those in the undiagnosed group and the control group.In the confirmed group,the relative expression levels of miR-3191-5p in the mild and moderate group were higher than those in the severe group,while BMD and BGP were lower than those in the severe group.Meanwhile,miR-3191-5p was obviously negatively correlated with both of BMD and BGP,respectively(P<0.05).The miR-3191-5p distinguished mildmoderate and non-mild-moderate,severe and non-severe,SANFH and non-SANFH patients,and the sums of areas under ROC were 0.764,0.916 and 0.911,respectively.Conclusion Serum miR-3191-5p can be used as a sensitive biomarker for early diagnosis of SANFH in patients.

关 键 词：激素股骨头坏死 微小RNA 骨密度 骨钙素 

分 类 号：R681.8[医药卫生—骨科学]