玉米转录因子ZmEREB180调控根系生长发育及耐逆的功能研究  

Functional study on the regulation of root growth and development and stress tolerance by maize transcription factor ZmEREB180

作　　者：刘宸铭 赵克勇 悦曼芳 赵延明[1] 吴忠义[2] 张春[2] LIU Chen-Ming;ZHAO Ke-Yong;YUE Man-Fang;ZHAO Yan-Ming;WU Zhong-Yi;ZHANG Chun(College of Agriculture,Qingdao Agricultural University,Qingdao 266109,Shandong,China;Institute of Biotechnology,Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology,Beijing 100097,China)

机构地区：[1]青岛农业大学农学院,山东青岛266109 [2]北京市农林科学院生物技术研究所/农业基因资源与生物技术北京市重点实验室,北京100097

出　　处：《作物学报》2024年第8期1920-1933,共14页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(32001430,32372053)资助。

摘　　要：AP2/ERF(APETALA2/ethylene-responsive factor)转录因子是植物中最大的转录因子家族之一,在调控植物生长发育、响应逆境胁迫、调节激素信号转导和物质代谢等多种生物学过程中发挥着重要作用。目前AP2/ERF超家族基因在许多植物物种中的生物学功能已经得到验证,但对玉米(Zea mays L.)中AP2/ERF基因的结构和功能的研究报道较少。前期工作中我们发现,ZmEREB180(ethylene-responsive element binding)转录因子,在鉴定的玉米六叶期(V6)、十二叶期(V12)和抽雄期(VT)等关键发育时期,根系中的表达量均存在显著差异;通过组织表达分析发现该基因主要在玉米根系中表达,且在幼根中的表达量显著高于成熟根,推测该基因可能参与玉米根系生长发育调控。本研究克隆了ZmEREB180(Gene ID:100192457)基因,结合生物信息学分析、实时荧光定量PCR(RT-qPCR)、亚细胞定位和转基因拟南芥(Arabidopsis thaliana L.)株系的耐逆表型鉴定等生物学手段,初步分析了该基因的表达模式和生物学功能。该基因包含2个外显子,编码序列全长1023 bp,编码340个氨基酸,具有AP2/ERF家族所特有的保守结构域;该基因在玉米根系中表达量最高,且在高盐、干旱、高氮和低氮等胁迫处理条件下的玉米根部皆有不同程度的诱导表达,其中,低氮处理较高氮处理具有更高的表达量和更快的响应速率;在含0.10、0.15 mol L^(-1)NaCl以及0.15、0.20和0.30 mol L^(-1)甘露醇(mannitol)的1/2 MS固体培养基上,转ZmEREB180基因拟南芥的主根长度均显著长于野生型;土壤环境中,高盐和干旱胁迫条件下的转基因植株比野生型拟南芥具有更健康的生长状态、更高的绿叶率、更低的丙二醛含量和更高的过氧化物酶活性。转录因子ZmEREB180可能在调控玉米根系生长发育方面具有积极的促进作用,并且能增强玉米植株对高盐、干旱、渗透、低氮等逆境胁迫的耐受性。本研究为下一步�The AP2/ERF(APETALA2/ethylene-responsive factor)family is one of the largest families of transcription factors in plants,and plays an important role in regulating a variety of biological processes such as plant growth and development,respondng to adversity stress,and regulating hormone signaling and substance metabolism.The biological functions of AP2/ERF family genes in many plant species have been validated,but fewer studies have been reported in maize(Zea mays L.).In the previous work,there was significant difference in the relative expression level of the ZmEREB180 transcription factor in root system between the identified critical developmental stages of maize at the six-leaf(V6),the twelve-leaf(V12),and tasseling(VT)stages,the tissue expression analysis revealed that this gene was mainly expressed in maize root system and was significantly higher in young roots than in mature roots,and it was hypothesized that this gene might be involved in the regulation of maize root growth and development.In this study,we cloned the ZmEREB180(Gene ID:100192457)transcription factor gene,and preliminarily analyzed the relative expression pattern and biological functions of ZmEREB180 by bioinformatics,RT-qPCR,subcellular localization,and stress-resistant phenotype identification of transgenic Arabidopsis(Arabidopsis thaliana L.)lines.This gene contained two exons and the full-length cDNA was 1023 bp,encoding 340 amino acids.The gene had a conserved domain unique to the AP2/ERF family,which expressed most highly in root system of maize;and the gene had different degrees of induced expression under high salt,drought,high nitrogen,and low nitrogen treatment conditions,with a more rapid and higher expression in response to low nitrogen than high nitrogen;root length of ZmEREB180 transgenic Arabidopsis lines were all significantly longer than the wild type(WT)on 1/2 MS medium containing 0.10 mol L^(-1),0.15 mol L^(-1)NaCl,and 0.15 mol L^(-1),0.20 mol L^(-1),0.30 mol L^(-1)mannitol(MNT).Under high salt and drought stress conditio

关 键 词：玉米 ZmEREB180 转录因子 根系 非生物胁迫 氮利用效率 

分 类 号：S513[农业科学—作物学] Q943.2[生物学—植物学]