OsRPTA18参与调控水稻叶片倾角的功能  

OsRPTA18 participated in the regulation of leaf inclination in rice

作　　者：何丹丹 舒亚洲 周海连 吴松果 魏晓双 杨明冲 李波[1,2] 吴正丹 韩世健 杨娟[2,3] 王继斌[1,2,4] 王令强 HE Dan-Dan;SHU Ya-Zhou;ZHOU Hai-Lian;WU Song-Guo;WEI Xiao-Shuang;YANG Ming-Chong;LI Bo;WU Zheng-Dan;HAN Shi-Jian;YANG Juan;WANG Ji-Bin;WANG Ling-Qiang(Guangxi University/State Key Laboratory of Conservation and Utilization of Agric-Biological Resources,Nanning 530004,Guangxi,China;College of Agriculture,Guangxi University,Nanning 530004,Guangxi,China;National Demonstration Center for Experimental Plant Science Education/Guangxi University,Nanning 530004,Guangxi,China;Moutai Institute,Renhuai 564501,Guizhou,China)

机构地区：[1]广西大学/亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004 [2]广西大学农学院,广西南宁530004 [3]植物科学国家级实验教学示范中心/广西大学,广西南宁530004 [4]茅台学院,贵州仁怀564501

出　　处：《作物学报》2024年第8期1934-1947,共14页Acta Agronomica Sinica

基　　金：广西自然科学基金面上项目(2022GXNSFAA035479);广西自然科学基金重点项目(2020GXN9)资助。

摘　　要：RPTA(regulatory particle triple-A ATPase)家族与植物的生长发育、激素调控和逆境胁迫反应密切相关。本研究一共鉴定到33个OsRPTA基因家族成员,并分析了其基因位置、基因结构、motif组成和启动子顺式作用元件等信息。随后,利用水稻CREP数据库下载的数据,分析了OsRPTA基因家族成员的全生育期组织表达模式。发现大部分OsRPTA基因在穗、胚乳和愈伤组织中具有较高的表达水平。β-D-葡萄糖苷酸酶(GUS)染色进一步显示,基因成员OsRPTA18主要在叶枕、根、叶、叶鞘、茎节、内稃和外稃的维管束等部位表达。亚细胞定位结果显示OsRPTA18蛋白定位于细胞核。通过CRISPR/Cas9基因编辑获得了突变体材料osrpta18-1和osrpta18-2。与中花11相比,突变体植株的株高、叶倾角变小,粒宽和千粒重降低。组织切片结果表明,突变体osrpta18旗叶倾角变小是由于叶枕近轴面厚壁细胞增殖,导致近轴面与远轴面细胞和维管束的不对称性减弱。本研究有助于了解水稻RPTA基因家族功能,并为利用OsRPTA18基因培育理想株型的水稻品种提供参考。The regulatory particle triple-A ATPase(RPTA)gene family is closely related to plant growth and development,hormone regulation and stress response.In this study,a total of 33 members of the gene family were identified in rice and their gene locations,gene structures,motifs composition,and cis-elements in the promoter regions were revealed.Then,the relative expression patterns of the gene members in OsRPTA family were investigated with the data downloaded from rice CREP database.Most of the OsRPTA genes expressed highly in panicle,endosperm and callus tissues.β-D-glucuronidase(GUS)staining further indicated that one of the members,OsRPTA18,was expressed in collar,root,leaf,leaf sheath,stem node,vascular bundles of glume and lemma.Subcellular localization indicated that OsRPTA18 protein was in nucleus.Two knock-out mutants(osrpta18-1 and osrpta18-2)were generated by CRISPR/Cas9 editing technology and both exhibited reduced plant height,leaf inclination,accompanied by the decreased grain width and 1000-grain weight compared to the wild type Zhonghua 11.Transverse section staining of the lamina joints of the plants indicated the changes in the cell wall thickness and vascular bundle size,causing the obvious proliferation of thick-walled cells at adaxial side,thus decreasing asymmetric development of the lamina joints and the leaf angel in the osrpta18 mutants.These results of this study are valuable for further functional analysis of RPTA genes and utilization of the OsRPTA18 gene for the genetic improvement of rice varieties with ideal plant architecture.

关 键 词：水稻 RPTA基因家族 叶枕 叶倾角 

分 类 号：S511[农业科学—作物学]