玉米N-乙酰转移酶ZmNAT1基因响应非生物胁迫的功能分析  

Functional analysis of maize N-acetyltransferase ZmNAT1 gene in response to abiotic stress

作　　者：郭思语 赵克勇 代正罡 邹华文[1] 吴忠义[2] 张春[2] GUO Si-Yu;ZHAO Ke-Yong;DAI Zheng-Gang;ZOU Hua-Wen;WU Zhong-Yi;ZHANG Chun(College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China;Institute of Biotechnology,Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology,Beijing 100097,China)

机构地区：[1]长江大学农学院,湖北荆州434025 [2]北京市农林科学院生物技术研究所/农业基因资源与生物技术北京市重点实验室,北京100097

出　　处：《作物学报》2024年第8期2001-2013,共13页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(32001430,32171952,31971839)资助。

摘　　要：GNAT(Gcn5-related N-acetyltransferase)家族蛋白在调控植物生长发育和响应逆境胁迫等过程中发挥着重要作用。目前GNAT家族基因在多个物种中的生物学功能已有报道,但在玉米(Zea mays L.)中的功能验证研究却很少。探究玉米GNAT家族基因的功能,不仅能丰富我国的玉米育种基因资源,同时也可为玉米的新种质资源创制提供重要依据。本研究克隆了ZmNAT1基因(Gene ID:541936,GRMZM2G123159),通过生物信息学分析发现,该基因CDS全长为519 bp,编码172个氨基酸,具有GNAT家族特有的保守结构域。通过对ZmNAT1基因在玉米不同时期不同组织中的表达量和不同逆境胁迫下表达模式分析发现:ZmNAT1在成熟根中的表达量最高,在不同非生物逆境胁迫处理下,ZmNAT1基因均有不同程度的诱导表达。通过异源表达获得了3株独立的表达量较高的转基因拟南芥(Arabidopsis thaliana L.)纯合株系,对其进行了不同逆境胁迫处理下表型鉴定实验,结果表明,转基因拟南芥相对于野生型拟南芥有更好的表型,在盐胁迫、渗透胁迫和干旱条件下的转基因株系的根显著长于野生型,且植株较野生型植株的绿叶率和叶绿素含量均升高、丙二醛含量降低,差异均达到显著水平。由此推测,ZmNAT1基因可能参与玉米对干旱、盐等非生物逆境胁迫的应答。本研究为进一步解析ZmNAT1在玉米中的生物学功能提供了重要的参考依据。The GNAT(Gcn5-related N-acetyltransferase)family proteins play a crucial role in regulating plant growth and development,and responding to stress.At present,the biological functions of GNAT family genes have been reported in many species,but there are few studies on its function validation in maize(Zea mays L.).Exploring the functions of maize GNAT family genes not only enriches the genetic resources for maize breeding in China,but also provides an important basis for the creation of new germplasm resources of maize.In this study,the ZmNAT1 gene(Gene ID:541936,GRMZM2G123159)was cloned.Bioinformatics analysis showed that the CDS of this gene was 519 bp,encoding 172 amino acids.ZmNAT1 contained a conserved domain unique to GNAT family.The relative expression level of ZmNAT1 gene in different tissues of maize at different stages under different stress conditions showed that the expression level of ZmNAT1 was the highest in mature roots,and the relative expression of ZmNAT1 gene could be induced in different degrees under different abiotic adversity stress conditions.Three independent transgenic Arabidopsis(Arabidopsis thaliana L.)pure lines with higher expression were obtained by heterologous expression,and phenotypic characterisation experiments under different adversity stress treatments were carried out on them,the results showed that transgenic Arabidopsis had a better phenotype relative to wild-type Arabidopsis,and the roots of transgenic plants under salt stress,osmotic stress,and drought were significantly longer than those of the wild-type,and the plants showed higher green leaf rate and chlorophyll content,and lower malondialdehyde content than the wild-type plants,which were significant difference.We speculated that ZmNAT1 gene may be involved in response to abiotic stress such as drought and salt in maize.This study provides an important reference for further analysis of the biological functions of ZmNAT1 in maize.

关 键 词：玉米 ZmNAT1 根系 生长发育 逆境胁迫 

分 类 号：S513[农业科学—作物学] Q943.2[生物学—植物学]