季节变化对仿刺参Toll样受体信号通路基因表达的影响  

Seasonal Variation in Expression of Genes from Toll-Like Receptor Signaling Pathway of Sea Cucumber Apostichopus japonicus

作　　者：肖瑶 高杉[1] 赵泽龙 蒋经伟[1] 周遵春[1] XIAO Yao;GAO Shan;ZHAO Zelong;JIANG Jingwei;ZHOU Zunchun(Key Laboratory of Germplasm Improvement and Fine Seed Breeding for Marine Aquatic Animals,Key Laboratory of Protection and Utilization of Aquatic Germplasm Resources,Ministry of Agriculture and Rural Affairs,Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China)

机构地区：[1]辽宁省海洋水产科学研究院,农业农村部水产种质资源保护与发掘利用重点实验室,辽宁省海洋水产动物种质改良与优良苗种繁育重点实验室,辽宁大连116023

出　　处：《水产科学》2024年第4期520-530,共11页Fisheries Science

基　　金：辽宁省科技计划项目(2022JH2/101300287,2021JH2/10200042);辽宁省百千万人才工程资助项目(2021921071);大连市支持高层次人才创新创业项目(2021RT08,2022RQ093);辽宁省农业科学院基本科研业务费计划项目(2022DD268143);辽宁省农业科学院学科绩效后补助项目(2022-HBZ-1007)。

摘　　要：为探明季节变化对仿刺参先天性免疫系统中的重要免疫通路——Toll样受体(TLR)信号通路相关基因表达的影响,2018年1—12月,每月中旬从辽宁省海洋水产科学研究院引育种中心的仿刺参单养池塘采集仿刺参样品9只,通过实时荧光定量PCR技术测定仿刺参TLR信号通路主要因子的转录表达水平,同时测定池塘的主要水环境参数,通过皮尔逊相关性分析方法分析仿刺参TLR信号通路主要因子的转录表达与环境因子季节性变化的相关性。试验结果显示,6月Toll、IRAK4、P105、TAK、IκKβ、Rel、TLR3、TBK及IRF8基因的表达水平较低,表明仿刺参体内TLR信号通路的应答能力在夏初较低。通过相关性分析发现,MyD88和TRAF6基因转录表达的季节性变化与溶解氧水平的季节性变化呈显著正相关、TAK基因和TBK基因转录表达的季节性变化与水环境的氧化还原电位呈显著正相关、IRF8基因转录表达与水温呈显著负相关、SARM基因转录表达与水温呈显著正相关,表明池塘环境因子的季节变化影响仿刺参TLR信号通路相关因子的表达。氧化还原电位影响MyD88依赖途径,且是关键环境因子,溶解氧是影响MyD88依赖途径的关键环境因子,而水温是影响MyD88非依赖途径的关键环境因子。In order to investigate the effect of seasonal variation on the expression of genes from Toll-like receptor(TLR) signaling pathway of sea cucumber Apostichopus japonicus,the transcriptional expression levels of major factors from TLR signaling pathway were detected in sea cucumber with body weight of(236±5) g sampled from a sea cucumber culture pond from introduce breeding center in Liaoning Ocean and Fisheries Science Research Institute in the middle of each month from January to December 2018 by qRT-PCR method,and the water environmental parameters in the pond were analyzed with handheld multiparameter meter for evaluation of the correlations between the seasonal variation of TLR-related genes expression levels and the environmental parameters by Pearson′s correlation test.The transcriptional expression analysis showed that there were low expression levels of Toll,IRAK4,TAK,P105,Rel,TLR3,TBK and IRF8 genes in June,indicating that TLR pathway was provided with low response ability in June.The correlation analysis showed that there was significantly positive correlation between MyD88 and TRAF6 genes expression and dissolved oxygen concentration,significantly positive correlation between TAK and TBK genes expression and redox potential,significantly negative correlation between IRF8 gene expression and water temperature,significantly positive correlation between SARM gene expression and water temperature,and significantly positive correlation between IκKβ gene expression and pHmv.The finding implied that the environmental factors led to important and complicated effects on the expression of TLR-related factors in the sea cucumber,among which the redox potential was key environmental factor for both MyD88-dependent and-independent pathways,the dissolved oxygen and pHmv were key environmental factors for MyD88-dependent pathway,and temperature was key environmental factor for MyD88-independent pathway.

关 键 词：仿刺参 TLR信号通路 MyD88依赖途径 MyD88非依赖途径 转录表达 季节性变化 

分 类 号：Q786[生物学—分子生物学]