低氧胁迫对四角蛤蜊存活、抗氧化指标和呼吸相关酶的影响  

Effects of Hypoxia Stress on Survival,Antioxidant Indices and Activities of Respiration-Related Enzymes of Surf Clam Mactra veneriformis

作　　者：于帅 宋依琳 陈丽梅 梁健 郭永军 YU Shuai;SONG Yilin;CHEN Limei;LIANG Jian;GUO Yongjun(Tianjin Key Laboratory of Aqua-Ecology and Aquaculture,College of Fisheries Science,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384

出　　处：《水产科学》2024年第4期606-613,共8页Fisheries Science

基　　金：财政部与农业农村部国家现代农业产业技术体系项目(CARS-48)。

摘　　要：为探究低氧胁迫对四角蛤蜊存活、抗氧化指标和呼吸相关酶的影响,设置溶解氧质量浓度分别为7.5(对照组)、4.0、2.0 mg/L处理组,测定分析四角蛤蜊的存活率、抗氧化指标(超氧化物歧化酶,过氧化氢酶和丙二醛)和呼吸相关酶(乳酸脱氢酶和丙酮酸激酶)的响应情况。试验结果显示:四角蛤蜊的存活率随着溶解氧质量浓度降低而降低,胁迫时间越长,死亡率越高;对照组在试验期间内存活率为100%;处理组在36 h后出现死亡个体,4.0 mg/L和2.0 mg/L试验组在48 h的存活率分别为97.5%和91.67%,在72 h的存活率分别为78.66%和67.30%。胁迫时间和溶解氧质量浓度对酶活性均有显著影响(P<0.05);对照组的酶活性于试验期间基本保持稳定;4.0 mg/L和2.0 mg/L试验组的抗氧化指标和呼吸相关酶总体呈现先升高后下降的趋势,肝胰腺的超氧化物歧化酶和过氧化氢酶活性在6 h达到最大值,显著高于其他时间点,鳃丝的丙二醛含量在12 h达到最大值;处理组鳃丝中的丙酮酸激酶活性均高于对照组。试验结果将有助于明确低氧胁迫对四角蛤蜊生理和生化活动的影响机制,为进一步探讨四角蛤蜊低氧耐受机制和创制耐低氧新种质提供参考资料。In order to probe the effects of hypoxic stress on the survival,antioxidant indices and respiration-related enzyme activities of surf clam Mactra veneriformis,the surf clam with shell length of(35.91±2.31) mm were put into a plastic box and exposed to dissolved oxygen concentrations of(2.0±0.2) mg/L and(4.0±0.2) mg/L by continuous nitrogen filling,and to dissolved oxygen concentration of(7.5±0.2) mg/L by continuous oxygenation(control group) at water temperature of(20±1) ℃.The survival,antioxidant indices(superoxide dismutase,catalase and malondialdehyde) and activities of respiration-related enzymes(lactate dehydrogenase and pyruvate kinase) were measured at 0,6,12,24,48 and 72 h of hypoxia stress.The results showed that the survival rate of surf clam was decreased with the decrease in dissolved oxygen concentration,and the stress period,with survival rate of 100% during the experiment in the control group,and dead individuals in 36 h in the treatment groups.There were survival rates of 97.5% in 48 hours in 4.0 mg/L group and 91.67% in 2.0 mg/L group,and at 72 hours the survival rates of 78.66% in 4.0 mg/L group and 67.30% in 2.0 mg/L group.The enzyme activities were significantly affected by stress time and dissolved oxygen concentration(P<0.05),and primarily stable during the experimental period in the control group.An overall trend of first increase and then decrease in antioxidant indices and activities of respiratory related enzymes was observed in 4.0 mg/L group and 2.0 mg/L group,with the maximal activities of superoxide dismutase and catalase in hepatopancreas at 6 h,significantly higher than those at other time points.The activity of pyruvate kinase in the gill filaments was found to be higher in the treatment groups,with the maximum content of malondialdehyde at 12 hours,than that in the control group.The findings will help to clarify the mechanism of the effects of hypoxia stress on the physiological and biochemical activities of surf clam,and provide a reference with further exploring the mech

关 键 词：四角蛤蜊 低氧胁迫 存活率 抗氧化指标 呼吸相关酶 

分 类 号：S968.317[农业科学—水产养殖]