维氏气单胞菌对斑马鱼胚胎发育的阻滞  

Inhibition of Embryonic Development of Zebrafish by Aeromonas veronii

作　　者：熊广泽 张怡晴 刘子琦 张铭琪 张焱 XIONG Guangze;ZHANG Yiqing;LIU Ziqi;ZHANG Mingqi;ZHANG Yan(Wuhan Foreign Languages School,Wuhan 430022,China;School of Life Sciences,Central China Normal University,Wuhan 430079,China)

机构地区：[1]武汉外国语学校,湖北武汉430022 [2]华中师范大学生命科学学院,湖北武汉430079

出　　处：《水产科学》2024年第4期630-639,共10页Fisheries Science

摘　　要：维氏气单胞菌是一种能感染人-兽-水生动物的革兰氏阴性病原菌。本试验分析了不同密度(2.2×10^(8)、1.1×10^(8)cfu/mL和5.5×10^(7)cfu/mL)的维氏气单胞菌对斑马鱼胚胎在受精后3 h到56 h胚胎形态学发育的影响及原因。通过形态学观察及数据统计、转录组分析和荧光定量PCR检测方法结果显示,维氏气单胞菌浸染斑马鱼胚胎后,不仅导致其死亡率增高,而且存活胚胎的发育显著延迟。维氏气单胞菌的密度越高,胚胎发育延迟的程度就越高,在密度为2.2×10^(8)cfu/mL的维氏气单胞菌感染下,斑马鱼胚胎发育时期阻滞在受精后8~14 h。转录组结果显示,维氏气单胞菌影响了斑马鱼胚胎与发育过程相关的基因表达,受影响的大部分基因的功能集中在中胚层的发育,尤其是胚胎的背腹侧分化中。本试验结果表明,维氏气单胞菌对斑马鱼发育具有一定的阻滞作用,原因可能是维氏气单胞菌改变了中胚层发育背腹侧分化相关基因的表达量或其上游调控因子活性。Zebrafish Danio rerio embryos that 3 h post fertilization were incubated in a Petri dish and added 50 mL of 0(clean water control),2.2×10^(8)cfu/mL(high concentration),1.1×10^(8) cfu/mL(medium concentration),and 5.5×10^(7) cfu/mL(low concentration) concentrations of Aeromonas veronii to soak the infection.The embryonic development was observed and taken photography under a bulk microscope at 6 h,24 h,32 h,48 h,56 h,72 h and 80 h for calculation of embryonic death and hatchability,to research on the effects of A.vitilii infection on early embryonic development morphology.The total RNA of the embryos was extracted at 6 h and 24 h for transcriptome sequencing.It was found that significant increase in mortality and delayed development of viable embryos were observed in the zebrafish embryos exposed to A.veronii,with the longer delaying in embryo development at higher concentration of A.veronii.Under the infection at a concentration of 2.2×10^(8) cfu/mL,the developmental period stagnated at 8—14 hours post fertilization(hpf).However,the embryos infected by A.veronii at 2.2×10^(8)cfu/mL were put back into clean water and continued to develop forward again.The transcriptome results of the changes in gene expressions related to the developmental process of the embryos exposed to A.veronii revealed that the 22 differentially expressed genes related to the developmental process were screened,most of whose functions were involved in the development of the mesoderm,especially in the embryonic dorsolization.So,we speculated that A.veronii changed the expression of genes related to the dorsolization of mesoderm development or the activity of upstream regulatory factors,resulting in the delayed development of zebrafish embryos.

关 键 词：维氏气单胞菌 斑马鱼 发育阻滞 胚胎 

分 类 号：S917.4[农业科学—水产科学]