微流控离子浓差极化芯片研制及其生化检测中的应用  

Development of Microfluidic Ion Concentration Polarization Chip and Its Application in Biochemical Testing

作　　者：贺志恒 王小丽 葛闯 徐溢[1,2] HE Zhi-Heng;WANG Xiao-Li;GE Chuang;XU Yi(Key Disciplines Laboratory of National Defense for Novel Micro-Nano Devices and System Technology,Key Laboratory of Optoelectronic Technology and Systems,Ministry of Education,Chongqing University,Chongqing 400044,China;School of Optoelectronic Engineering,Chongqing University,Chongqing 400044,China;School of Life and Health Information Science and Engineering,Chongqing University of Posts and Telecommunications,Chongqing 400065,China;Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment,Chongqing University Cancer Hospital,Chongqing 400030,China)

机构地区：[1]重庆大学,新型微纳器件与系统技术国防重点学科实验室&光电技术与系统教育部重点实验室,重庆400044 [2]重庆大学光电工程学院,重庆400044 [3]重庆邮电大学生命健康信息科学与工程学院,重庆400065 [4]重庆大学肿瘤医院,肿瘤转移转化与个体化治疗重点实验室,重庆400030

出　　处：《生物化学与生物物理进展》2024年第7期1618-1631,共14页Progress In Biochemistry and Biophysics

基　　金：国家重点研发计划(2020YFB2009001);国家自然科学基金(62071072);重庆市医学科研重点攻关项目(重庆市卫健委、科技局联合项目)(2023GGXM002);智能传感与微纳生化系统(2019研究生导师团队)项目资助。

摘　　要：离子浓差极化(ion concentration polarization,ICP)现象是在外加电场作用下发生在微纳交界面处的一种电富集现象,将ICP现象与微流控分析技术相结合,可广泛应用于生化分析中带电粒子预富集、目标物分离、靶标物检测等领域。本文首先对ICP原理及微流控ICP芯片进行了简要介绍,梳理总结了ICP芯片的制备技术和方法,其中重点关注了微流道结构设计、纳米结构制备与设计等方面的研究现状与进展。首先对基础单通道ICP芯片的结构进行分析,进而对并行通道ICP芯片结构以及集成多功能的微流控ICP芯片进行了总结和讨论,列举了ICP芯片中纳米结构的制备方法及其优缺点。进而,讨论了优化ICP芯片的富集效能途径,可通过引入多场耦合、阀门控制等多种手段,实现对靶标物的富集效能优化。最后,针对ICP芯片在多种带电生化样本分析检测中的应用进行综述,指出ICP芯片在匹配检测目标生物特性方面面临挑战,需要提高富集效率和选择性,解决流体控制、混合及传输问题。可以看到,微流控ICP芯片具有处理样本流量低、分离富集效果好、检测效率高以及易于集成化和小型化等优势,在生化检测领域展示出很好的研究意义和实用前景。Ion concentration polarization(ICP)is an electrical transport phenomenon that occurs at the micronano interface under the action of an applied electric field,and the ICP phenomenon can be used to enrich charged particles with high efficiency.The microfluidic chip has the advantages of high precision,high efficiency,easy integration and miniaturization in biochemical analysis,which provides a new solution and technical way for biochemical analysis.In response to the demand for the detection of trace charged target analytes in sample solution,the advantages of high enrichment multiplicity,convenient operation and easy integration of ICP are utilized to provide an effective way for microfluidic biochemical detection.The combination of ICP phenomenon and microfluidic analysis technology has been widely used in the fields of pre-enrichment of charged particles,separation of targets,and detection of target analytes in biochemical analysis.In this paper,the principle of ICP and the microfluidic ICP chip are briefly introduced.Under the action of external electric field,the co-ions pass through the ion-selective nanochannel,the counterions are rejected at the boundary of nanochannel to form a depletion zone,and the charged samples will be enriched at the boundary of the depletion zone.Then the preparation techniques and methods of ICP chips are summarized.Among them,the design of microfluidic channel structure and the preparation and design of nanostructures are emphasized.The basic single-channel structure is analyzed,and the parallel-channel structure as well as the integrated multi-functional microfluidic ICP chip are sorted out and summarized.The preparation methods of nanostructures in ICP chips and their respective advantages and disadvantages are listed,and it is summarized that the current mainstream means are the embedding method and the self-assembly method,and attention is paid to the design of nanostructures preparation methods by both of them.In addition,this paper also discusses how to optimize the enrichme

关 键 词：离子浓差极化 微纳结构 微流控芯片 分离富集 生化检测 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] Q5-3[自动化与计算机技术—控制科学与工程] O65[生物学—生物化学]