对氯苯丙氨酸建立失眠模型行为学评价方法的研究  

Study on Behavioral Evaluation Method for Establishing Insomnia Model with Para-chlorophenylalanine

作　　者：曲子涵 马海钊 张野[1] 刘英豪 郅晓宇 李宝杰 张红石[1] QU Zihan;MA Haizhao;ZHANG Ye;LIU Yinghao;ZHI Xiaoyu;LI Baojie;ZHANG Hongshi(Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学,长春130117

出　　处：《实验动物科学》2024年第3期9-13,共5页Laboratory Animal Science

基　　金：国家自然科学基金项目-面上项目(82074569);2021年大学生创新创业训练计划项目(S202110199081)。

摘　　要：目的 通过观察对氯苯丙氨酸(PCPA)失眠大鼠模型在造模成功后一周内,各类行为学实验中的学习记忆能力改变,验证PCPA失眠模型稳定性。方法 30只SD大鼠随机均分为空白组和模型组,空白组正常饲养,对模型组大鼠腹腔注射PCPA建立失眠模型,建模后第1周进行Morris水迷宫实验等5项大鼠行为学监测。结果 与空白组比较,模型组大鼠睡眠潜伏期显著延长(P<0.01),睡眠持续时间显著缩短(P<0.01);在水迷宫实验中,模型组大鼠寻台时间延长(P<0.01);巴恩斯迷宫实验中,模型组大鼠测试探索期的目标洞口停留总时间显著缩短(P<0.01);避暗实验中,模型组错误次数增多(P<0.05),且潜伏期显著延长(P<0.01);高架十字迷宫实验中,模型组大鼠开臂停留时间显著缩短(P<0.01);旷场实验显示模型组大鼠在中央区域的停留时间、直立次数和运动总距离均低于空白组,其中中央区域的停留时间具有显著差异(P<0.01)。结论 PCPA失眠模型具有较高的可重复性和稳定性,可以作为睡眠和记忆相关研究的基础模型。Objective By observing the changes in learning and memory abilities in various behavioral experiments of the para-chlorophenylalanine(PCPA)insomnia rat model within a week after successful modeling,the stability of the PCPA insomnia model was verified.Method Thirty SD rats were randomly divided into blank group and model group,the rats in the blank group were fed normally,and establishment of insomnia model by intraperitoneal injection of PCPA into model group rats.Five behavioral tests including Morris water maze test were performed in the first week after modeling.Result Compared with the blank group,the sleep latency of the model group was significantly prolonged(P<0.01),and the sleep duration was significantly shortened(P<0.01).In the water maze experiment,the time of seeking platform in the model group was prolonged(P<0.01).In the Barnes maze experiment,the total residence time at the target portal of the model group rats during the test exploration period was significantly shortened(P<0.01).In the dark avoidance experiment,the number of errors in the model group increased(P<0.05),and the latency was significantly prolonged(P<0.01).In the elevated cross maze experiment,the open arm residence time of rats in the model group was significantly shortened(P<0.01).In the open-field experiment,the dwell time,the number of uprightness and the total distance of movement in the central area of the model group were all lower than those of the blank group,among which the dwell time in the central area was significantly different(P<0.01).Conclusion The PCPA insomnia model has high reproducibility and stability,and can be used as a basic model for sleep and memory-related studies.

关 键 词：PCPA 失眠模型 行为学评价 

分 类 号：Q95-3[生物学—动物学]