金黄色葡萄球菌对实验动物的感染及其分子分型方法比较  

Comparison on Molecular Typing Technologies and the Infection Condition of Staphylococcus Aureus in Laboratory Animals

作　　者：吴运谱 张泽辉 崔子寅 梁喜才 王杏 塔铭骏 王春田[1] WU Yunpu;ZHANG Zehui;CUI Ziyin;LIANG Xicai;WANG Xing;TA Mingjun;WANG Chuntian(College of Laboratory Animal Medicine,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;College of Traditional Chinese Medicine,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)

机构地区：[1]辽宁中医药大学实验动物医学院,沈阳110847 [2]辽宁中医药大学中医学院,沈阳110847

出　　处：《实验动物科学》2024年第3期85-89,共5页Laboratory Animal Science

基　　金：辽宁省民生科技计划项目(2021JH2/10300032);辽宁中医药大学博士科研启动项目(2100221004);辽宁中医药大学大学生创新创业训练计划项目(X202210162017)。

摘　　要：金黄色葡萄球菌作为一种人畜共患的条件性病原菌,其可对实验动物的质量及从业人员的健康构成严重的威胁,是屏障环境中必须监测和排除的病原体。采用准确、灵敏、快速的细菌分子分型方法,对于控制屏障环境中金黄色葡萄球菌具有重要意义。目前,金黄色葡萄球菌的分子分型方法有葡萄球菌染色体基因盒mec,葡萄球菌主蛋白A分型、多位点系列分型、脉冲场凝胶电泳分型、附属基因调节因子分型等。了解屏障系统中金黄色葡萄球菌对实验动物的感染状况,比较了不同分子分型方法的技术特征与应用,可为实验动物金黄色葡萄球菌的检测及分型提供参考。Staphylococcus aureus(S.aureus)is a zoonotic opportunistic pathogen,which is must be monitored and excluded from the barrier environment and often is a serious threat to the quality of laboratory animals and the health of practitioners.It is critical to use accurate,sensitive,and rapid method of molecular typing to control the transmission of S.aureus in the laboratory animal barrier environment.The molecular method for typing S.aureus main including Staphylococcal cassette chromosome mec typing,Staphylococcal protein A typing,multilocus sequence typing,Pulsed field gel electrophoresis typing,accessory gene regulator typing,and others.Understand the infection status of Staphylococcus aureus in laboratory animals of barrier systems and comparation of the technical characteristics and applications of different molecular typing method,which can provide a reference for the detection and typing of Staphylococcus aureus of laboratory animal origin.

关 键 词：实验动物 金黄色葡萄球菌 感染 分子分型 

分 类 号：Q95-3[生物学—动物学]