北京平谷区桃细菌性穿孔病病原菌鉴定及其拮抗菌筛选  

Identification of Pathogen Causing Bacterial Shot Hole of Peaches in Pinggu District of Beijing and Screening of Its Antagonistic Microorganisms

作　　者：孙前军 杨丽丹 王睿琦 杨海清 卢彩鸽 刘建斌 杨俊刚 梁子安[1] 王甲辰 张殿朋 SUN Qianjun;YANG Lidan;WANG Ruiqi;YANG Haiqing;LU Caige;LIU Jianbin;YANG Jungang;LIANG Zian;WANG Jiachen;ZHANG Dianpeng(Nanyang Normal University,Nanyang 473061,China;Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;The Fruit Office of Pinggu District in Beijing,Beijing 101200,China)

机构地区：[1]南阳师范学院,南阳473061 [2]北京市农林科学院,北京100097 [3]北京市平谷区果品办公室,北京101200

出　　处：《中国生物防治学报》2024年第3期661-669,共9页Chinese Journal of Biological Control

基　　金：北京市农林科学院科技创新能力建设专项(KJCX20230207);国家重点研发计划(2022YFE0199500)。

摘　　要：为筛选对平谷区桃穿孔病有抑制作用的菌株,对桃穿孔病病原菌进行DNA测序鉴定后筛选拮抗菌。应用组织分离法从平谷区发病桃果实分离桃穿孔病原菌并进行致病性测定,根据菌株形态特征和16S rDNA序列相似率分析,初步确定病原菌为树生黄单胞菌桃李致病变种Xanthomomonas arboricola pv. pruni。采用平板对峙法筛选出病原菌的拮抗菌5株,其中菌株xj39抑菌效果最为显著,抑菌圈直径达到54.7 mm,菌株xj12次之,抑菌圈直径50.8 mm;经16S rDNA序列和rpoB基因序列比对及相似率分析,确定拮抗菌株xj39为大孢子链霉菌Streptomyces fimbriatus,菌株xj12为生二素链霉菌Streptomyces ambofaciens。田间防验结果表明,拮抗菌xj39发酵液对桃细菌性穿孔病防治效果为33%,与化学药剂春雷霉素处理无显著差异。To obtain strains that inhibit bacterial perforation of peach in Pinggu District,we screened antagonistic bacteria after identifying the pathogen through DNA sequencing.The peach perforating pathogen was isolated from morbid peach fruits in Pinggu District using a tissue isolation method and pathogenicity was determined.Based on morphological characteristics and 16S rDNA sequence similarity analysis,the pathogen was preliminarily identified as Xanthomonas arboricola pv.pruni.Five bacterial strains had antagonistic activity using the plate standoff method.Among them,strain xj39 exhibited the most significant inhibitory effect,with a diameter of the inhibition zone reaching 54.7 mm.The second was strain xj12,with a diameter of the inhibition zone of 50.8 mm.16S rDNA and rpoB gene sequences analysis indicated that strain xj39 was Streptomyces fimbriatus and strain xj12 was Streptomyces ambofaciens.Field prevention test showed that the fermentation solution of xj39 had the control efficacy of 33%against peach bacterial perforation disease,which was not significantly different from that of the chemical kasugamycin treatment.

关 键 词：桃穿孔病 病原菌 黄单胞菌 拮抗菌 菌株鉴定 

分 类 号：S476[农业科学—农业昆虫与害虫防治]