十字花科根肿病拮抗菌贝莱斯芽胞杆菌ZF72分离鉴定及生防效果研究  

Identification and Biocontrol Effect of Bacillus velezensis ZF72 against Clubroot disease of Crucifers

作　　者：林子涵 张思雨 康华军 袁晓伟 李兴盛 石延霞[1] 谢学文[1] 李磊 范腾飞 李宝聚[1] 柴阿丽[1] LIN Zihan;ZHANG Siyu;KANG Huajun;YUAN Xiaowei;LI Xingsheng;SHI Yanxia;XIE Xuewen;LI Lei;FAN Tengfei;LI Baoju;CHAI Ali(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,State Key Laboratory of Vegetable Biobreeding,Beijing 100081,China;College of Horticulture,China Agricultural University,Beijing 100193,China;Shandong Huasheng Agriculture Limited Company,Qingzhou 262500,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081 [2]中国农业大学园艺学院,北京100193 [3]山东省华盛农业集团股份有限公司,青州262500

出　　处：《中国生物防治学报》2024年第3期717-726,共10页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2022YFD1401200);中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS);大宗蔬菜产业技术体系(CARS-25)。

摘　　要：针对十字花科根肿病日益严重且防治困难的问题,采用平板对峙法,以尖孢镰孢菌Fusarium oxysporum和辣椒疫霉菌Phytophthora capsici为指示菌进行初筛,通过盆栽试验复筛,得到十字花科根肿病高效生防菌株ZF72,盆栽防效74.6%。根据形态学特征、生理生化特性及16S rDNA,gyr B和atp D多基因系统发育分析,鉴定菌株ZF72为贝莱斯芽胞杆菌Bacillus velezensis。采用单因素试验对菌株ZF72摇瓶发酵工艺进行优化,确定最佳配方为山梨醇3 g/L、麸皮8 g/L、CaCl_(2)1 g/L和MgCl_(2)·6H_(2)O 3 g/L,最适发酵条件为温度28℃、pH 6.0、装液量50 mL/250 mL、转速240 r/min、种子液接种量1%(v/v)。田间试验结果显示,菌株ZF72处理根肿病症状明显减轻,田间防效可达63.8%,为根肿病生物防治提供了新的微生物资源。Clubroot disease of cruciferous plants caused by Plasmodiophora brassicae Woronin occurred severely and was difficult to control.In this study,a biocontrol agent ZF72 was screened by using dual-culture assay against Fusarium oxysporum and Phytophthora capsica.Control efficacy of ZF72 against clubroot disease in pot experiment was 74.6%.The strain ZF72 was identified as Bacillus velezensis based on morphological characteristics,physiological and biochemical analysis,as well as 16S rDNA,gyrB and atpD phylogenetic analysis.The fermentation of strain ZF72 was optimized by single factor test.An optimum medium composition was as followings:sorbitol 3 g/L,bran 8 g/L,CaCl_(2)1 g/L,and MgCl_(2)·6H_(2)O 3 g/L.The optimum fermentation conditions were as followings:temperature 28℃,pH 6.0,loading volume 50 mL/250 mL,rotating speed 180 r/min,and inoculation amount 1%(v/v).Treatment with strain ZF72 significantly reduced the incidence of clubroot disease of Chinese cabbage, with a control efficacy of 63.8% in field experiment, which provides new microbial resources for biological control of clubroot disease of crucifers.

关 键 词：十字花科根肿病 贝莱斯芽孢杆菌 生物防治 

分 类 号：S476[农业科学—农业昆虫与害虫防治]