血小板裂解液在大鼠脊髓损伤中的治疗作用  

Therapeutic effect of platelet lysate on spinal cord injury in rats

作　　者：王玲 金红旭 刘颖 陈哲远 项良碧 WANG Ling;JIN Hong-xu;LIU Ying;CHEN Zhe-yuan;XIANG Liang-bi(Department of Emergency,General Hospital of Northern Theater Command,Shenyang Liaoning 110001,China;Department of Orthopedics,General Hospital of Northern Theater Command,Shenyang Liaoning 110001,China)

机构地区：[1]北部战区总医院急诊科,辽宁沈阳110001 [2]北部战区总医院骨科,辽宁沈阳110001

出　　处：《局解手术学杂志》2024年第7期566-570,共5页Journal of Regional Anatomy and Operative Surgery

基　　金：辽宁省科技厅项目(2020JH2/10300157)。

摘　　要：目的验证血小板裂解液(PL)对大鼠脊髓损伤的治疗作用。方法将30只雄性SD大鼠随机分为假手术组、损伤组、治疗组,每组10只。假手术组大鼠暴露脊髓后不予砸击;损伤组大鼠暴露脊髓后以20 g自制打击器砝码从5 cm高度垂直落下砸击脊髓,持续5 s,制作大鼠脊髓损伤模型;治疗组大鼠建立脊髓损伤模型后给予腹腔注射PL 2 mL,每日1次,连续19 d。采用脚印分析(BBB)评分及斜坡实验评定大鼠后肢运动功能;每组随机取6只大鼠,于第7天分别取大鼠脊髓进行HE染色及电镜扫描,观察其病理改变。结果术后第3、7、14天,3组大鼠BBB评分差异有统计学意义(P<0.05),且治疗组BBB评分高于损伤组(P<0.05)。治疗组、损伤组在术后第7、14天斜坡实验结果有显著性差异(P<0.01);HE染色结果显示,治疗组与损伤组比较神经元变性坏死减少,细胞肿胀改善,小胶质细胞吞噬现象减少;电镜结果显示,治疗组与损伤组比较星形胶质细胞胞质水肿及细胞膜局部破损均减轻,线粒体肿胀、变性等减轻。结论PL能够改善脊髓损伤大鼠后肢运动功能及减轻脊髓损伤后病理改变,对于大鼠脊髓损伤有治疗作用。Objective To verify the therapeutic effect of platelet lysate(PL)on spinal cord injury in rats.Methods Thirty male SD rats were randomly divided into the sham operation group,the injury group and the treatment group,with 10 rats in each group.Rats in the sham operation group did not smash the spinal cord after exposure;rats in the injury group were established the rat spinal cord injury model by smashing the spinal cord with a 20 g of self-made smashing machine weights from a height of 5 cm for 5 seconds after exposure;rats in the treatment group were given intraperitoneal injection of 2 mL of PL once a day for 19 days after established the spinal cord injury model.The hind limb function was evaluated by Basso Beattie Bresnahan(BBB)score and slope experiment;six rats were randomly selected from each group,and the spinal cords of the rats were taken on the 7th day after surgery for HE staining and electron microscopy scanning to observe their pathological changes.Results There were statistically significant differences in BBB scores on the 3rd,7th and 14th days after surgery among the three groups (P<0. 05), and the BBB scores of the treatment group were higher than those of the injury group (P<0. 05). There were statistically significant differences in the results of slope experiment on the 7th and 14th days after surgery between the treatment group and the injury group (P<0. 01). HE staining showed that the neuronal degeneration and necrosis decreased, the cell swelling improved and the microglial phagocytosis decreased in the treatment group compared with those in the injury group. The results of electron microscopy showed that the astrocyte cytoplasmic swelling and local cell membrane damage were alleviated,and the mitochondrial swelling and degeneration in the treatment group were relieved compared with those in the injury group. Conclusion PL can improve the hind limb function and reduce the pathological changes after spinal cord injury in rats, which has therapeutic effect on spinal cord injury in rats.

关 键 词：脊髓损伤 血小板裂解液 脚印分析 斜坡实验 

分 类 号：R459.9[医药卫生—治疗学] R651.2[医药卫生—临床医学]