等离子体光纤光栅超痕量汞离子传感器  

Plasmonic Fiber Grating Sensors for Ultra-Trace Mercury Ion Detection

作　　者：王芳[1] 廉岚淇 卢梦迪 张扬[2] Wang Fang;Lian Lanqi;Lu Mengdi;Zhang Yang(School of Optoelectronic Engineering,Taiyuan University of Technology,Taiyuan 030600,Shanxi,China;School of Physics,Dalian University of Technology,Dalian 116024,Liaoning,China)

机构地区：[1]太原理工大学电子信息与光学工程学院,山西太原030600 [2]大连理工大学物理学院,辽宁大连116024

出　　处：《光学学报》2024年第9期258-266,共9页Acta Optica Sinica

基　　金：国家自然科学基金(62205232);山西省基础研究计划资助项目(202203021212250)。

摘　　要：为实现特异性、超痕量、原位的重金属汞离子检测,提出一种基于适配体和磁性纳米粒子增强倾斜光纤布拉格光栅-表面等离激元共振(TFBG-SPR)汞离子传感器。首先,在倾斜光纤布拉格光栅包层区域溅射厚度合适的金膜以激发表面等离激元共振,在探针末端镀金从而形成反射式传感结构。然后,采用具有胸腺嘧啶(T)碱基失配的适配体修饰TFBG-SPR栅区,进行特异性汞离子识别。最后,选择链霉亲和素包被的磁性纳米粒子作为信号放大标签,以提高离子检测性能。汞离子与T碱基作用激活适配体序列,连接磁性纳米粒子,传感器表面折射率发生变化,TFBG-SPR包层模式的振幅产生响应。结果表明:采用差分幅度解调,传感器对汞离子的检测极限为0.5 pmol/L,检测范围为1 pmol/L~10μmol/L,所提传感器具备良好的特异性以及再生性,同时通过测量临床样品的回收率,可以证实其在即时、快速检测领域中的应用前景。Objective Mercury(Hg)is a volatile and highly toxic heavy metal element.Under the action of atmospheric deposition and ocean circulation,mercury ions(Hg2+)easily enter fresh water or the ocean,causing harm to the ecological environment and human body.It is of great significance to develop a high-sensitivity mercury ion detection system for environmental protection and human health.In recent years,various methods for the detection of mercury ions have been developed,including atomic absorption spectrometry,fluorescent probe method,solid-phase extraction,and inductively coupled plasma optical emission spectrometry techniques.However,these methods often require sophisticated instruments,specialized operators,and time-consuming procedures.In addition,some of these methods are prone to interference from other metal ions,which can lead to false positive results.DNA biosensor research has taken center stage in the past decade,marking a significant advancement in heavy metal ion detection.Mercury ions selectively bind to two thymidines(T).During the hybridization of DNA molecules,mercury ions can change the structure of some double helix bases of DNA and form a T—Hg2+—T mismatch structure,which is more stable than the base pair.The specific detection of mercury ions as a heavy metal can be realized by using the T—T mismatch mechanism of DNA molecules.However,it still faces the problems of low sensitivity,long response time,and low accuracy.Therefore,the demand for rapid and precise mercury ion detection in clinical settings and environmental pollutant monitoring necessitates innovative approaches.Methods In order to achieve specific,ultra-trace,and in situ mercury ion detection,a tilted fiber Bragg grating-surface plasmon resonance(TFBG-SPR)biosensor based on aptamer and magnetic nanoparticles(MNPs)enhancement is designed.TFBG-SPR has a rich mode field distribution and narrow linewidth,which enables the high-performance detection of physical changes and chemical reactions without being affected by the external envi

关 键 词：传感器 光纤传感器 光纤布拉格光栅 表面等离激元 痕量离子检测 

分 类 号：TN253[电子电信—物理电子学]