血站实验室抗-TP检测情况分析  

Analysis of anti-TP detection in blood center laboratories

作　　者：王鲜媛 赵雅姿 王艳彬[1] 李聪雅 唐晰 韩卫[1] WANG Xianyuan;ZHAO Yazi;WANG Yanbin;LI Congya;TANG Xi;HAN Wei(Hebei Blood Center,Shijiazhuang,050071,China)

机构地区：[1]河北省血液中心,石家庄050071

出　　处：《临床血液学杂志》2024年第6期401-404,共4页Journal of Clinical Hematology

摘　　要：目的了解河北省血液中心无偿献血人群梅毒感染的情况与特征。方法对血液中心2022年实验室抗-TP双试剂阳性标本的确证结果进行统计分析。结果2022年共检测标本203097人份,来自165876例献血者,抗-TP ELISA筛查不合格率为0.16%(334/203097),双试剂阳性率为0.10%(202/203097),双试剂阳性标本的梅毒螺旋体颗粒凝集试验(TPPA)确证阳性率为0.08%(167/203097),估算流行率约为0.10%(167/165876)。TPPA阳性标本中金标阳性占比97.60%(163/167)。初次献血者确证阳性率(0.23%)高于重复献血者(0.02%)(P<0.01),女性献血者流行率(0.13%)高于男性(0.08%)(P<0.01)。初次献血者中性别比较差异无统计学意义(P>0.05),女性确证阳性率为0.26%,男性为0.21%;年龄组比较差异有统计学意义(P<0.01),26~35岁年龄段确证阳性率最低(0.16%),56~60岁最高(0.87%)。重复献血者中TP每年的新发感染率为0.01%。共筛查到4例TP合并HIV感染者。结论本中心无偿献血人群梅毒确证阳性率较低。在不具备条件进行TPPA确证试验的情况下,用双试剂阳性率去推算较不合格率更接近于梅毒真实感染水平。如在献血前初筛项目中增加抗-TP胶体金法能够拦截绝大部分梅毒感染者和部分合并HIV感染者,减少不必要的资源浪费。Objective To understand the situation and characteristics of Treponema pallidum(TP)infection in the unpaid blood donation population of our center.Methods Conduct statistical analysis on the confirmation results of anti-TP ELISA dual-reagent reactive specimens from unpaid blood donors in our center in 2022.Results In 2022,a total of 203097 samples were detected from 165876 blood donors.The unqualified rate of anti-TP ELISA screening was 0.16%(334/203097),and the dual-reagent reaction rate was 0.10%(202/203097).The positive rate of TPPA was 0.08%(167/203097)and the estimated prevalence was about 0.10%(167/165876).97.60%(163/167)of TPPA positive samples were gold standard positive.The confirmed positive rate of first-time blood donors(0.23%)was higher than that of repeated blood donors(0.02%)(P<0.01),and the prevalence rate of female blood donors(0.13%)was slightly higher than that of male blood donors(0.08%)(P<0.01).There was no significant difference in gender among first-time blood donors(P>0.05).The positive rate was 0.26%for females and 0.21%for males.There was statistical difference in age group(P<0.01),the lowest positive rate was found in 26-35 years old(0.16%),and the highest rate was found in 56-60 years old(0.87%).The annual rate of new TP infections among repeated donors was 0.01%.A total of 4 cases of TP with HIV infection were detected.Conclusion The confirmed positive rate of syphilis among unpaid blood donors in Shijiazhuang area was relatively low.In the absence of the conditions for the confirmatory test of TPPA,the dual-reagent reaction rate was used to estimate that the unqualified rate was closer to the true level of syphilis infection.If the anti-TP colloidal gold method was added to the pre-donation screening project,the vast majority of syphilis infections and some HIV-infected patients could be intercepted,and the unnecessary waste of resources could be reduced.

关 键 词：无偿献血者 梅毒螺旋体 合并感染 

分 类 号：R446.6[医药卫生—诊断学]