患者首次输血后短期内产生不规则抗体致血型正反定型不一致分析  

Analysis on the forward and reverse blood typing inconsistency caused by irregular antibodies after patients'first blood transfusion in the short term

作　　者：贲燕华[1] 王峻霞 夏和凤[1] 韩霜[1] BEN Yanhua;WANG Junxia;XIA Hefeng;HAN Shuang(Department of Blood Transfusion,People's Hospital of Dongtai,Dongtai,224200,China)

机构地区：[1]东台市人民医院输血科,江苏东台224200

出　　处：《临床血液学杂志》2024年第6期429-431,436,共4页Journal of Clinical Hematology

摘　　要：目的分析患者首次输血后短期内产生不规则抗体对血型正反定型的影响,正确鉴定患者血型和不规则抗体特异性,保证临床输血安全。方法采用试管法、凝聚胺法、微柱凝胶卡式法进行不规则抗体筛选、抗体鉴定试验、Rh分型及直接抗人球蛋白试验分析不规则抗体特异性;2-巯基乙醇(2-ME)处理标本后鉴定血型。结果患者血型为B型Rh阳性,Rh分型为ccDEE,血清中检出IgM、IgG抗-C。结论患者首次输血后即可产生具有临床意义的特异性不规则抗体,建议对献血者和患者进行Rh血型系统抗原(CcEe)分型,可以快速匹配抗原相合血液,避免因抗体筛查阳性引起交叉配血困难而延误患者的输血治疗,减少不规则抗体引起的溶血性输血反应的发生。Objective To analyze the impact of irregular antibodies produced by patients on forward and reverse typing among short term after their first blood transfusion,and accurately identify the patient's blood type and the specificity of irregular antibodies,as well as ensure clinical blood transfusion safety.Methods Test-tube,polyamine and microcolumn-gel-card methods were applied for irregular antibody screening,antibody identification,Rh typing and direct-anti-human-globulin test,to analyze the specificity of irregular antibodies;blood type was identified after 2-ME treatment.Results The patient's blood type was B type Rh positive,Rh subtype was ccDEE,and IgM and IgG anti C were detected in the serum.Conclusion After the first blood transfusion,specific irregular antibodies with clinically significance were produced by patients.It was recommended to perform group system antigen(CcEe)typing on Rh blood for blood-donors and patients,that could make quick blood matching with common antigen,avoid transfusion treatment delay due to difficulty from cross matching caused by positive antibody screening,and reduce the occurrence of hemolytic transfusion reactions caused by irregular antibodies.

关 键 词：不规则抗体 抗-C IgG IGM 

分 类 号：R457.1[医药卫生—治疗学]