β-氨基丁酸诱导枸杞抗根腐病的响应机制  

Response mechanism of root rot resistance of wolfberry induced byβ-aminobutyric acid

作　　者：孙涛 李捷[1,2] 张建金 李栋[1] 张煦[1] 李永晖 SUN Tao;LI Jie;ZHANG Jianjin;LI Dong;ZHANG Xu;LI Yonghui(College of Forestry,Gansu Agricultural University,Lanzhou,Gansu 730070,China;Wolfberry Harmless Cultivation Engineering Research Center of Gansu Province,Lanzhou,Gansu 730070,China;Agricultural Technology Extension Center of Jingyuan County,Jingyuan,Gansu 730699,China)

机构地区：[1]甘肃农业大学林学院,甘肃兰州730070 [2]甘肃省枸杞无害化栽培工程研究中心,甘肃兰州730070 [3]甘肃省靖远县农业技术推广中心,甘肃靖远730699

出　　处：《西北农林科技大学学报（自然科学版）》2024年第8期101-111,121,共12页Journal of Northwest A&F University(Natural Science Edition)

基　　金：甘肃省2022科技攻关项目(22CX8ND151);甘肃省高校创新基金项目(No.2020A-055)。

摘　　要：【目的】探究β-氨基丁酸诱导枸杞抗根腐病的生理机制,为枸杞根腐病的高效防治提供参考。【方法】分别以β-氨基丁酸诱导的枸杞根系和清水灌根的枸杞根系(对照)为材料,针刺接种尖孢镰刀菌,分别在接种后0和128 h测定枸杞根腐病病斑面积,计算病情指数并提取枸杞根系代谢物。通过代谢组学方法分析诱导组和枸杞-镰刀菌互作组枸杞根系中的差异代谢物,结合多元统计分析方法解析代谢物的变化情况。【结果】经β-氨基丁酸诱导的枸杞根系在接种尖孢镰刀菌128 h时枸杞根腐病的病斑面积和病情指数显著低于对照组。代谢组学共筛选出79种显著上调差异代谢物和94种显著下调差异代谢物。多元统计分析结果显示,诱导组和互作组组内的重复样本相关性极强、重复性好,可以用于后续差异代谢物分析。KEGG富集分析发现,诱导组中氨酰基-tRNA生物合成通路存在显著差异代谢物富集,互作组中氨酰基-tRNA生物合成和氰基氨基酸代谢通路存在显著差异代谢物富集。代谢物热图分析结果显示,诱导组中甘氨酸、组氨酸、脯氨酸、天冬氨酸、谷氨酸、天门冬酰胺和色氨酸含量分别上调了211.5%,206.1%,182.5%,176.5%,118.0%,106.7%和75.2%;互作组中β-氰基丙氨酸、天门冬氨酸、天门冬酰胺、异亮氨酸、酪氨酸、缬氨酸和苯丙氨酸含量分别上调了440.5%,403.6%,304.1%,219.2%,185.9%,185.2%和169.5%。【结论】氨酰基-tRNA生物合成和氰基氨基酸代谢通路可能是β-氨基丁酸诱导枸杞抗根腐病的关键代谢途径。【Objective】This study explored physiological mechanism ofβ-aminobutyric acid-induced resistance to root rot in wolfberry to provide reference for efficient control of root rot in wolfberry.【Method】The roots of wolfberry induced byβ-aminobutyric acid and those irrigated with clear water were inoculated with Fusarium oxysporum by acupuncture method.The lesion area was measured at 0 and 128 h after inoculation,the disease index was calculated and the metabolites were extracted.The differential metabolites in roots of wolfberry in induction group and wolfberry-Fusarium interaction group were analyzed by metabonomic method,and the changes of metabolites were analyzed by multivariate statistical analysis.【Result】The lesion area and disease index of wolfberry roots induced byβ-aminobutyric acid were significantly lower than those of the control group at 128 h after inoculation with F.oxysporum.A total of 79 up-regulated differential metabolites and 94 down-regulated differential metabolites were obtained.The multivariate statistical analysis showed that repeated samples in the induction group and interaction group had strong correlation and good repeatability,which could be used for subsequent differential metabolite analysis.KEGG enrichment analysis showed that there were significant differences in aminoacyl-tRNA biosynthesis pathway in induction group and significant differences in aminoacyl-tRNA biosynthesis and cyanoamino acid metabolism pathway in interaction group.The metabolite heat map analysis showed that glycine,histidine,proline,aspartic acid,glutamic acid,asparagine and tryptophan in the induction group were up-regulated by 211.5%,206.1%,182.5%,176.5%,118.0%,106.7%and 75.2%,respectively.In the interaction group,β-cyanoalanine,aspartic acid,asparagine,isoleucine,tyrosine,valine and phenylalanine were increased by 440.5%,403.6%,304.1%,219.2%,185.9%,185.2%and 169.5%,respectively.【Conclusion】Aminoacyl-tRNA biosynthesis and cyano-amino acid metabolic pathway were key metabolic pathways inβ-am

关 键 词：枸杞根腐病 Β-氨基丁酸 代谢组学分析 诱导抗性 代谢通路 

分 类 号：S435.671[农业科学—农业昆虫与害虫防治]