视频+实践在心血管外科硕士研究生制备小鼠心肌肥厚模型教学中的应用  

Application of video-teaching plus practice in teaching mice model preparation of myocardial hypertrophy by graduate students of cardiovascular surgery

作　　者：马继鹏 李子林 王晓武 张帅帅 王晓亚 许富杰 方魏逸 刘金成 MA Ji-peng;LI Zi-lin;WANG Xiao-wu;ZHANG Shuai-shuai;WANG Xiao-ya;XU Fu-jie;FANG Wei-yi;LIU Jin-cheng(Department of Cardiovascular Surgery,First Affiliated Hospital,Air Force Medical University,Xi’an 710032,Shaanxi,China)

机构地区：[1]空军军医大学第一附属医院心血管外科,陕西西安710032

出　　处：《心脏杂志》2024年第4期486-491,共6页Chinese Heart Journal

基　　金：陕西省科技资源开放共享平台项目(2023-CX-PT-06);国家自然科学基金项目(82070264,82270420);陕西省重点研发计划项目(2022ZDLSF01-09)。

摘　　要：目的比较视频+实践教学法与常规教学法在心外科研究生建立心肌肥厚动物模型的教学效果。方法选取2021级心外科5名硕士研究生为常规组,选取2022级心外科5名硕士研究生为视频组,两组学生分别以常规示教和视频示教进行教学。纳入研究小鼠210只,其中正常对照组10只、常规组学生和视频组学生每组各制备100只模型小鼠。比较常规组和视频组的麻醉、气管插管、主动脉弓缩窄、术后苏醒等围术期的相关数据,以及术后4周存活率和模型成功率,从而评价视频教学法和常规教学法在模型制备教学上的效果。结果通过视频教学法的研究生能够反复观看老师示教,熟练掌握各个环节操作,渡过学习曲线。与常规组研究生相比较,视频组的研究生在麻醉、插管时间、手术时间、缝合时间、术后苏醒时间等指标上均显著低于常规组的研究生(P<0.01);视频组研究生在制备模型围手术期的各个环节动物死亡数显著低于常规组研究生,术后4周动物存活率、模型成功率显著高于常规组研究生(P<0.01)。结论由于心血管疾病动物模型操作难度大、过程较为复杂,利用视频教学法对硕士研究生进行示教与教学,视频教学组研究生能够更快掌握手术能力,获得操作技巧,克服限速步骤,能够制备成功率高、重复性强的心血管疾病动物模型,缩短学习曲线和时间,能够为进一步研究相关疾病的病理机制、治疗等提供条件。AIM To evaluate the teaching effect of video-teaching plus practice in mice model preparation of myocardial hypertrophy by graduate students of cardiovascular surgery.METHODS Two hundred and ten mice were used for this study,including 10 mice in control group,100 mice in routine training group and 100 mice in video-teaching group.Compared to routine teaching,the teachers’demonstrations were recorded as videos,so the trainees in video-teaching group can watch the video repeatedly during the practice process.Perioperative related data such as anesthesia,tracheal intubation and aortic constriction,postoperative recovery,the 4-week postoperative survival rate and model success rate were compared between routine training group and video-teaching group.RESULTS The trainees in video-teaching group were more proficient in various aspects of operation.The anesthesia,intubation time,surgical time,suture time and postoperative recovery time in video-teaching group were significantly lower than those in routine training group.The number of animal deaths during the perioperative period of model preparation in video-teaching group was also significantly lower than that in routine training group,and the survival rate and model success rate at the 4^(th) week after surgery in video-teaching group were significantly higher than those in routine training group.CONCLUSION The preparation and operation of cardiovascular disease animal models is difficult and the process is relatively complex.However,video-teaching can shorten the time of learning curve,which helps graduate students better master surgical skills and prepare cardiovascular disease animal models with high success rates and strong repeatability.

关 键 词：视频教学 动物模型 心血管疾病 研究生 围手术期 

分 类 号：R542.2[医药卫生—心血管疾病]