育龄期妇女子宫内膜息肉中γδT细胞的表达及临床意义  

Investigation the association betweenγδT cells with endometrial polyps

作　　者：孟子晗 杨雨露 邹慧娟 张雪珂[1,4,6,7] 杨文娟 孙东莹 许孝凤 Meng Zihan;Yang Yulu;Zou Huijuan;Zhang Xueke;Yang Wenjuan;Sun Dongying;Xu Xiaofeng(Dept of Obstetrics and Gynecology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract,Hefei 230032;Key Laboratory of Population Health Across Life Cycle,Ministry of Education,Hefei 230032;Engineering Research Center of Biopreservation and Artifical Organs,Ministry of Education,Hefei 230032;Anhui Province Key Laboratory of Reproductive Health and Genetics,Hefei 230032;Biopreservation and Artificial Organs,Anhui Provincial Engineering Research Center,Anhui Medical Uiversity,Hefei 230032;Anhui Provincial Institute of Translational Medicine,Hefei 230032;The First Clinical Medical College of Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学第一附属医院妇产科,合肥230032 [2]国家卫生健康委配子及生殖道异常研究重点实验室,合肥230032 [3]出生人口健康教育部重点实验室,合肥230032 [4]生命资源保存与人工器官教育部工程研究中心,合肥230032 [5]生殖健康与遗传安徽省重点实验室,合肥230032 [6]安徽省生命资源保存与人工器官工程技术研究中心(安徽医科大学),合肥230032 [7]安徽省转化医学研究院,合肥230032 [8]安徽医科大学第一临床医学院,合肥230032

出　　处：《安徽医科大学学报》2024年第6期971-976,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金青年项目(编号:32000642);安徽省卫健委重点项目(编号:AHWJ2023A10019);安徽省重点研发与开发计划项目(编号:202204295107020041)。

摘　　要：目的探究子宫内膜息肉(EP)中γδT细胞和相关炎症因子的表达及其临床意义。方法研究对象为因EP不孕的患者(EP组,n=57)和非子宫因素的不孕症患者(Control组,n=44)。分别取EP组织和正常子宫内膜组织。使用独立样本t检验或Wilcoxon秩和检验评价两组一般特征,使用免疫荧光法观察增殖期子宫内膜和子宫内膜息肉中CD3和TCRγδ阳性细胞的表达,使用ELISA检测组织匀浆中白细胞介素(IL)-17和干扰素(IFN)-γ的表达水平,使用简单线性回归分析EP中γδT细胞的表达情况与相关各炎症细胞因子的相关性。结果EP组织中γδT细胞数目明显增加,炎性因子IL-17和IFN-γ水平也明显升高,并呈线性关系(P<0.05)。结论γδT细胞数目与EP导致的不孕症有关,提示可能通过调控细胞亚群失衡影响炎症因子的表达导致了胚胎植入失败。Objective To investigate the expression and clinical value ofγδT cells and related inflammatory factors in endometrial polyps(EP).Methods Subjects were patients with infertility due to EP(EP group,n=57)and infertility due to non-uterine factors(Control group,n=44).EP tissues and normal endometrial tissues were taken,respectively.The general characteristics of the two groups were evaluated by independent samples T-test or Wilcoxon rank sum test.The expressions of CD3 and TCRγδin proliferative endometrium and endometrial polyps were observed by immunofluorescence,and the expressions of IL-17 and IFN-γin tissue homogenate were detected by ELISA.Uni-variable linear regression was used to analyze the correlation between the number ofγδT cells and the expression level of IL-17 and IFN-γ.Results Both the number ofγδT cells and he levels of IL-17 and IFN-γsignificantly increased in endometrial polyp tissues,and the relationship between is linear(all P<0.05).ConclusionγδT cells are associated with EP among infertile women,which may lead to embryo implantation failure by affecting the expression of inflammatory factors and regulating the imbalance of cell subsets.

关 键 词：ΓΔT细胞 子宫内膜息肉 IL-17 IFN-Γ 炎症反应 胚胎植入失败 不孕症 

分 类 号：R711.74[医药卫生—妇产科学]