光学相干成像及临床应用研究  

Optical Coherence Imaging for Clinical Applications

作　　者：尹子辰 何滨 陈政宇[1,2] 胡章伟 石叶炅 薛睿智 杨攀琦 应雨哲 孟哲 荆林凯 王贵怀 薛平 Yin Zichen;He Bin;Chen Zhengyu;Hu Zhangwei;Shi Yejiong;Xue Ruizhi;Yang Panqi;Ying Yuzhe;Meng Zhe;Jing Linkai;Wang Guihuai;Xue Ping(State Key Laboratory of Low-Dimensional Quantum Physics and Department of Physics,Tsinghua University,Beijing 100084,China;Frontier Science Center for Quantum Information,Beijing 100084,China;Department of Neurosurgery,Beijing Tsinghua Changgung Hospital,School of Clinical Medicine and Institute of Precision Medicine,Tsinghua University,Beijing 102218,China)

机构地区：[1]清华大学物理系低维量子物理国家重点实验室,北京100084 [2]量子信息前沿科学中心,北京100084 [3]北京清华长庚医院神经外科,清华大学临床医学院,精密医学研究所,北京102218

出　　处：《中国激光》2024年第9期16-28,共13页Chinese Journal of Lasers

基　　金：国家自然科学基金(69908004,90508001,61227807,61575108);国家高技术研究发展计划(2006AA02Z472);国家重点基础研究发展计划(2009CB929400)。

摘　　要：精准识别肿瘤边界对于提高切除率、降低复发率、改善患者预后具有极其重要的意义,但目前仍缺乏术中及时、精准识别肿瘤边界及肿瘤侵袭性的成像方式。光学相干层析成像(OCT)作为一种无创、无标记、高分辨率的三维光学层析成像技术,不但可以获得组织的三维断层形貌特征,还能实现组织的三维微循环网络可视化。光学活性快速病理成像是一种不需要对术中新鲜组织进行任何预处理即可获得类似病理切片信息的高分辨率高对比度光学相干成像技术。本文将介绍推进光学相干成像在术中应用的系列研究工作,包括自适应肿瘤血管算法、机器人辅助OCT、显微镜集成OCT、超高速扫描激光器、术中实时三维OCT成像以及光学活性快速病理成像的研究和开发,以实现在肿瘤切除前获取肿瘤血管及其微循环信息,肿瘤切除后通过光学活性快速病理成像进行快速病理检测。这些研究将有利于提高肿瘤全切率、降低肿瘤复发率、改善胶质瘤患者预后,推进精准医学的发展。Significance Optical coherence tomography(OCT)plays a pivotal role in medical imaging,particularly in enhancing the tumor resection accuracy.The significance of this technology lies in its ability to improve patient prognosis by providing real-time,detailed visualization of tumor boundaries and invasiveness,thereby reducing recurrence rates and aiding the precise removal of malignant tissues.Progress We introduced a series of research efforts to advance the intraoperative application of optical coherence imaging.Vascular characteristics are an important basis for intraoperative pathological assessment.We first introduced OCT angiography with adaptive multi-time intervals,which proposes a time-efficient scanning protocol by adaptive optimization of the weights of different timeinterval B-scan angiograms.This novel OCTA technique achieved better performance,with a visible vascular density increase of approximately 67%and a signal-to-noise ratio enhancement of approximately 11.6%(Figs.2 and 3).In the context of intraoperative applications,we introduced robot-assisted OCTA,which integrated a high-resolution OCT system with a 6-degree of freedom robotic arm(Fig.4).Robot-assisted OCTA can achieve wide-field imaging of artificially determined scanning paths.High-resolution vascular imaging of the mouse brain by robot-assisted OCTA successfully confirmed the effect of unevenly distributed resolution and fall-off caused by the large-curvature sample(Fig.5).Thereafter,we introduced a microscope-integrated OCT system that can be well integrated with current intraoperative equipment and does not need to pause the surgical process(Figs.6 and 7).Providing real-time tissue depth information to a doctor can help improve their decision-making ability in delicate surgical procedures such as ophthalmology and nervous system surgery.Intraoperative three-dimensional(3D)real-time imaging requires an OCT system with high imaging and processing speeds.Thereafter,we introduced the 10.3 MHz ultra-high speed scanning laser with stretch pul

关 键 词：成像系统 无标记成像 光学相干断层扫描成像 术中病理 精准医疗 

分 类 号：O436[机械工程—光学工程]