表面增强拉曼光谱技术在流行性病毒检测领域的研究进展  

Research Progress on Epidemic Virus Detection Based on Surface-Enhanced Raman Spectroscopy

作　　者：刘艺 王楠 何绍华 张竣 冯尚源 林多 Liu Yi;Wang Nan;He Shaohua;Zhang Jun;Feng Shangyuan;Lin Duo(Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education,College of Photonic and Electronic Engineering,Fujian Normal University,Fuzhou 350007,Fujian,China)

机构地区：[1]福建师范大学光电与信息工程学院医学光电科学与技术教育部重点实验室,福建福州350007

出　　处：《中国激光》2024年第9期86-113,共28页Chinese Journal of Lasers

基　　金：国家自然科学基金(12374405)。

摘　　要：近几年新型冠状病毒COVID-19的迅速传播,引起了全球对传染病防控和快速病毒检测技术的高度关注。表面增强拉曼光谱(SERS)作为一种光学分析技术,凭借其独特的分子指纹特性和高检测灵敏度的特点,成为生物医学检测领域的有力工具,对可能大规模暴发的流行性病毒灵敏迅速的检测以及监控提供新颖、高效的光学解决方案。本文对从2021年以来开展的DNA、RNA病毒,尤其是威胁人类生命健康的流行性病毒检测工作当中使用的标记、非标记SERS技术进行梳理,从SERS基底结构建构及功能化修饰,分子探针的设计,高速响应、高灵敏度检测模型构建,生物技术、机器学习方法的联合使用等方面,特别是基于便携式、手持式拉曼光谱仪的研究,对SERS技术在病毒检测领域的应用进展进行了总结和展望。Significance e Viruses are the primary cause of many infectious diseases,including influenza,high-mortality lower respiratory tract infections,diarrhea,tuberculosis,HIV infection,dengue fever,hepatitis B,and more.These diseases can cause severe damage to various systems in the human body and can even lead to life-threatening conditions.The outbreak of infectious viruses poses a significant challenge to public healthcare systems.Early and accurate virus diagnosis is crucial in preventing virus spread,especially in the absence of specific vaccines or effective medications.Existing traditional detection methods often require complex equipment and the expertise of skilled operator.Hence,it becomes challenging to conduct large-scale testing in rapidly spreading virus-infected areas.Surface-enhanced Raman scattering(SERS)technology is an ultra-sensitive vibrational spectroscopy technique,which is used to detect plasmonic nanostructures on the surface or near-surface molecules.Due to its fast response,strong specificity,and non-invasive detection characteristics,SERS has been widely used in surface and interface studies,chemical and biosensors,biomedical monitoring,trace analysis,electrochemical reactions,and catalytic reactions.Specifically,in virus detection,it exhibits extremely high detection sensitivity,enabling rapid and accurate detection of minute virus particles.Based on the analysis of virus spectral features,SERS technology can differentiate between different types of viruses,including subtypes and variants.This high specificity leads to a unique advantage in virus tracing,classification,and epidemiological research,which is crucial for the rapid screening of early virus infections and facilitating timely medical intervention.This review systematically summarizes the research progress and potential applications of SERS technology in virus detection over the past two years,considering factors such as the genetic material of the virus,virus types,and the extent of impact(Fig.1).Progress Initially,this study cat

关 键 词：医用光学 表面增强拉曼光谱 病毒检测 生物传感器 纳米光子学 纳米医学 

分 类 号：O657.37[理学—分析化学]